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RacE null mutant divides when attached to a substrate. From A. Lozanne, U. Texas, Austin. |
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RacE null mutant aborts cytokinesis in suspension. From A. Lozanne, U. Texas, Austin. |
Digitized wild-type cell in chemotaxis
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Three dimensional direct image reconstruction of a D. discoideum amobae crawling in a gradient of cAMP. The chemoattractant source is left of the field. From D. Wessel and D. Soll, Unv. of Iowa |
Digitized clathrin heavy chain null cell in chemotaxis
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Three dimensional direct image reconstruction of a clathrin heavy chain null Dictyostelium cell. Note the aberrant cell shape, lack of polarity and numerous fragmented pseudopods. From D. Wessel and D. Soll, Univ. of Iowa. |
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D. discoideum cells expressing GFP-tagged to a highly conserved actin binding domain (ABD) crawling on a glass surface. DIC and fluorescence images were collected simultaneously. Images were captured every 10 seconds. From K.M. Pang, E. Lee, and K.A. Knecht, University of Connecticut. |
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Side view of amoebae expressing GFP-ABD plated on a glass surface. DIC and fluorescence images were collected simultaneously every 10 seconds by confocal microscopy. From E. Shelden and D.A. Knecht, University of Connecticut.
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Supported by NIH (NIGMS and NHGRI)
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