Additional Materials

The Dicty Stock Center accepts antibodies, cDNA and genomic libraries, and other materials people would like to share. The cost for most items on this page is $ 40.-, for GWDI pooled libraries it is $ 100.- for customers of non-profit institutions.

If you would like to send additional materials, or would like to obtain the materials listed below, please contact dictystocks@northwestern.edu.

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DNA libraries

• GWDI pooled library, REMI-seq project Thompson and Harwood labs

  Each GWDI pool is $100.-; the pools are a limited resource. Therefore, we would like each lab to order the resource not more than once. It is key when hatching/thawing to follow instructions for growing and storage of further aliquots. The REMI-seq Handbook is also available.



• Genomic Library pools from the Shaulsky lab

  Details of the genomic library can be found in Rosengarten et al. 2015. A 96-well plate with 32 pools of the library in TSA Big Easy E. coli, will be shipped on dry ice.

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Antibodies

• Monoclonal antibodies (MUD 1, MUD 3, MUD 4, MUD 9, MUD 5, MUD 10) generated against prespore and prestalk cell membrane proteins during the slug stage. 10mg/ml solutions in 1 ml aliquots.
  The antibodies were given by M. Krefft to Salvo Bozzaro who deposited to the DSC. References: Gregg et al. 1982, Krefft et al. 1983
  Some are published and described in detail:
• MUD 1 recognizes a cell surface antigen of prespore cells (Krefft et al. 1984, Krefft et al. 1985)
• MUD 3 recognizes a protein on the surface of spores (Voet et al. 1985)
• MUD 9 labels prestalk cells but also vegetative cells (Krefft et al. 1989, Krefft et al. 1985)
• Anti-calmodulin monoclonal antibodies (calA). Deposited by Margaret Clarke.
  There are several mouse monoclonal with different specificities (see paper for details): 2D1, 1F11, 4C5, 6D4, 20B9, and 15E7. Certain of those monoclonals are also availablel from Sigma and other commercial sources. Immunoblotting calmodulin requires special techniques, as described in the paper.
  Reference: Hulen et al. 1991.
• Anti-calmodulin polyclonal antiserum (calA). Deposited by Margaret Clarke.
  These antisera (B88, B89) probably cross-react with other calmodulin-like proteins in Dictyostelium. Reference: Zhu et al. 1993.
• Anti-cluA monoclonal antibody (cluA). Deposited by Margaret Clarke.
  The 10D5 mouse monoclonal antibodies. Reference: Zhu et al. 1997.
• Anti-myosin II heavy chain monoclonal antibodies (mhcA). Deposited by Margaret Clarke.
  There are four different mouse monoclonals: M342, M151, M98 and M27. M342 binds specifically to monomeric myosin in solution, presumably because the epitope is not accessible in thick filaments.
  References: Reines & Clarke 1985a, Reines & Clarke 1985b.
• Anti-ribosomal protein L7 antibody (rpl7). Reference: Szymkowski et al. 1989.
• Anti-discoidin-I polyclonal antiserum (dscA). Deposited by Margaret Clarke.
  Antisera from two different rabbits (Y355, Y356). Reference: Rathi and Clarke 1992.
• Anti-vatA monoclonal antibody (vatA). Deposited by Margaret Clarke.
  The N1 and N4 mouse monoclonal antibodies were generated by Agnes Fok at the University of Hawaii. Reference: Fok et al. 1993.
• Anti-vatM monoclonal antibody (vatM). Deposited by Margaret Clarke.
  The N2 mouse monoclonal antibodies were generated by Agnes Fok at the University of Hawaii. Reference: Fok et al. 1993.
• Anti-dimB antibody (dimB). Deposited by Yoko Yamada, Jeff Williams
  Raised in Rabbit; use at a titre of 1/5000. References: Zhukovskaya et al. 2006 and Yamada et al. 2013
• Anti-dimB-phosphoS5090P antibody (dimB). Deposited by Yoko Yamada, Jeff Williams
  Raised in Sheep; use at a titre of 1/300. Reference: Fukuzawa et al. 2001.
• Anti-statc-total-7H3 antibody (dstC). Deposited by Tsuyoshi Araki, Jeff Williams
  Raised in Mouse; use at a titre of 1/500 – 1/1000. Reference: Fukuzawa et al. 2001.
• Anti-statc-phospho-CP22 antibody (dstC). Deposited by by Tsuyoshi Araki, Jeff Williams
  Raised in Mouse; use at a titre of 1/300 – 1/500. Reference: Fukuzawa et al. 2001.

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Monoclonal antibodies are also available from the Developmental Studies Hybridoma Bank where you can view the list of Antibodies against Dictyostelium discoideum.