Strain Catalog

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Strain Descriptor Strain Summary Genotype Phenotype
lvsA-lvsA null mutant (Large Volume Sphere A)axeA1, axeB1, axeC1, pyr5-6-[pRG24], lvsA-[pEKKO-1], ura+axe
lvsA-lvsA REMI mutantaxeA1, axeB1, axeC1, lvsA-
lvsA-/lvsA(1-3348):GFPknock-in of lvsA with the 271 amino acid carboxyl terminus replaced with GFP axe, lvsA(1- 3348):GFP, bsR
lvsA-/[act15]:GFP:lvsA(2889-3619)lvsA-null overexpressing the C terminus of lvsA fused to GFPaxe, lvsA-, [act15]:lvsA(2889-3619), neoR
lvsA-/[act6]:GFP:lvsAknock-in of GFP-lvsA under the control of the act6 promoteraxe, lvsA-, [act6]:GFP:lvsA, bsR
lvsA-/[act6]:GFP:lvsA(1828- 3619)knock-in of GFP-lvsA with a 1828 amino acid deletion at the amino terminus under the control of the act6 promoteraxe, lvsA-/[act6]:GFP:lvsA(1828- 3619), bsR
lvsA-/[act6]:GFP:lvsA(659- 3619)knock-in of GFP-lvsA with a 658 amino acid deletion at the amino terminus under the control of the act6 promoteraxe, lvsA-, [act6]:GFP:lvsA(659- 3619), bsR
lvsB-lvsB null mutant (Large Volume Sphere B)axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-axe, lvsB-[lvsB-KO], bsRaxe,bsR
lvsB-lvsB null mutant (Large Volume Sphere B)axe, lvsB-[lvsB-KO], bsRaxe,bsR
lvsB-/[act15]:FLAG:Rab14(N121I)inactive rab14 in lvsB nullaxe, lvsB-, [lvsB-KO], FLAG:Rab14(N121I), bsR, neoR
lvsB-[act6]:TAP:GFP:lvsBknock-in of lvsB TAP-tagged and fused to GFP at the N-terminus under the control of the act6 promoteraxe, lvsB-[lvsB-KO], act6:TAP:GFP:lvsB, bsR
lvsC-lvsC null mutant (Large Volume Sphere C)axe, lvsC-[lvsC-KO], bsR
lvsD-lvsD null mutant (Large Volume Sphere D)axe, lvsD-[lvsD-KO], bsRaxe,bsR
lvsE-lvsE null mutant (Large Volume Sphere E)axe, lvsE-[lvsE-KO], bsRaxe,bsR
lvsF-lvsF null mutant (Large Volume Sphere F)axe, lvsF-[lvsF-KO], bsRaxe,bsR
lyC2-lyC2 null mutant in AX2axeA2, axeB2, axeC2, lyC2-, [bsR-loxP], bsR
M. luteusbacterial strain Micrococcus luteus; biosafety level 1 (USA)m.luteus
M2α-mannosidase-1 mutant; does not grow in plain HL5axeA1, axeB1, axeC1, manA2axenic,wt
M2-88/X9heterozygote diploid tester strain; parents: M2-88 and X9tsgI/+, +/cycA1, matA1/matA1, axeA1/+, axeC1/+, +/acrA1, +/tsgD12, +/whiA1, +/axeB1, +/bwnA1, manA2/+
M28Temperature-sensitive grower with round spores derived from a brown strain, which was used to demonstrate parasexual recombination in Dictyostelium.bwn, tsgE13, spr
M3-11/X9heterozygote diploid tester strain; parents: M3-11 and X9tsgI/+, +/cycA1, matA1/matA1, axeA1/+, axeC1/+, +/acrA1, +/tsgD12, +/whiA1, +/axeb1, manA3+
M31glucosidase II mutant (modA-)axeA1, axeB1, axeC1, modA1axe,wt
M31m31
M4-3isolated using the p-nitrophenyl-alpha-D-mannopyranoside assay; haploid tester strainmatA1, axeA1, axeC1, axeB1, manA4, tsg3
macA-macA null mutant in KAX3axeA1, axeB1, axeC1, macA-, bsR
macA-/[rpl11]:macAmacA null rescued with macA under the control of the rpl11 (V18) promoteraxeA1, axeB1, axeC1, macA-, [rpl11]:macA, bsR, neoR
malA-malA disrupted at aa 14axeA1, axeB1, axeC1, malA-, bsR
matA-matA null mutant in the temperature sensitive strain HM140 with the bsR cassette removedaxeA1, axeB1, axeC1, tsgA1, cyc-905, matA-, [bsR-loxP]
matA-matA null mutant in AX2; fails to form macrocysts in sexual crossesaxeA2, axeB2, axeC2, matA-, [bsRcas], bsR
MB35AX2 cells containing the transactivator plasmid of tet on/off system (pMB35)axeA2, axeB2, axeC2, tTAs*[pMB35], neoR, tetR
MBP-mlcBmyoB light chain N-terminal MBP (maltose-binding protein) fusion in AX3 axeA1, axeB1, axeC1, [MBP-mlcB], bsRaxe,bsR
med25-med25 REMI mutant; the insertion site is 1068 bp downstream of the 5'''' end of the third exon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: taataaCATGccaat;
get raw sequence data
axeA1, axeB1, axeC1, med25-, bsR
mef2A-mef2A null mutant in AX2 backgroundaxeA2, axeB2, axeC2, mef2A-, [bsR-loxP], bsR
mef2A-mef2A null mutant in AX4 backgroundaxeA1, axeB1, axeC1, mef2A-, [bsR-loxP], bsR
mef2A-/[ecmA]:lacZmef2A null mutant expressing lacZ under the control of the prestalk ecmA promoter.axeA1, axeB1, axeC1, mef2A-, [bsR-loxP], lacZ, [pEcmA-Gal], bsR, neoR
mef2A-/[ecmB]:lacZmef2A null mutant expressing lacZ under the control of the prestalk ecmB promoter.axeA1, axeB1, axeC1, mef2A-, [bsR-loxP], lacZ, [pEcmB-Gal], bsR, neoR
mef2A-/[pspA]:lacZmef2A null mutant expressing lacZ under the control of the prespore pspA promoter.axeA1, axeB1, axeC1, mef2A-, [bsR-loxP], lacZ, [pPsA-i-alpha-gal], bsR, neoR
mekA-mekA null mutant in JH10axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, mek1-[mek1-KO], thy+axe
mekA-mekA null in kAX3axeA1, axeB1, axeC1, mekA-, bsR
mekA-/ppp4C-mekA-/ppp4C- double null mutantaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, mek1-[mek1-KO], thy+, ppp4C-[pppC-KO], bsR
mekA-/ppp4C-/[act15]:FLAG:pppCFLAG-tagged ppp4C overexpressed in the mekA-/ppp4C- double null mutantaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, mek1-[mek1-KO], thy+, ppp4C-[pppC-KO], bsR, FLAG:ppp4C, [pEXP4(+)], neoR
mekA-/smkA-mek1/smkA double null mutantaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, mek1-[mek1-KO], thy+, smkA-[smkA-KO], bsRaxe,bsR
mekA-/[act15]:mekA(299-360)mekA- null mutant overexpressing mekA with an N-terminal deletion of the region upstream from the catalytic core under the control of the actin 15 promoteraxeA1, axeB1, axeC1, mekA-, [act15:mekA(299-360)], neoR
mekA-/[act15]:mekA(K321A)mekA- null mutant overexpressing mekA with the lysine residue required for ATP binding converted to an alanine (K321A) under the control of the act15 promoteraxeA1, axeB1, axeC1, mekA-, [act15/mekA(K321A)], neoR
mekA-/[act15]:mekA(S444A/T448A)mekA null mutant overexpressing mekA with double alanine substitutions (S444A/T448A) under the control of the act15 promoteraxeA1, axeB1, axeC1, mekA-, [act15:mekA(S444A/T448A)], neoR
mekA-/[act15]:mekA(S444E/T448E)mekA null mutant overexpressing a constitutively active form of mekA with double glutamate substitutions (S444E/T448E) under the control of the act15 promoteraxeA1, axeB1, axeC1, mekA-, [act15/mekA(S444E/T448E)], neoR
mekA-/[act15]:Myc:erkAmekA null mutant expressing Myc-tagged erkAaxeA1, axeB1, axeC1, mekA-, act15:Myc:erkA, bsR, neoR
mfeA-mfeA null mutant (peroxisomal multifunctional enzyme; MFE1)axeA2, axeB2, axeC2, mfeA-[mfeA-KO], bsRaxe,bsR
mfsd8-Genome Wide Dictyostelium Insertion bank (GWDI) mfsd8- mutant; insertion at position 1412784, chr 2; used enzyme: DpnIIaxeA1, axeB1, axeC1mfsn8-, [bsRcas], bsR
mgp1-mgp1 null mutantaxeA1, axeB1, axeC1, bsR, mgp1-
mgp1-/mgp2-mgp1/mgp2 double null mutant axeA1, axeB1, axeC1, brR, hygR, mgp1-, mgp2-
mgp2-mgp1 null mutant, blasticidin resistantaxeA1, axeB1, axeC1, brR, mgp2-
mgp2-mgp1 null mutant, hygromycin resistantaxeA1, axeB1, axeC1, hygR, mgp2-
mhcA(1-33/GTG/79-761)Partially N-terminally truncated (residues 34 to 78 deleted) motor domain of mhcA (N-terminal 761 residues) expressed in wild typeaxeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+, mhcA(1-33/GTG/79-2116) [pM761-deltaN2], neoR
mhcA(81-761)N-terminally truncated (residues 1-80 deleted) motor domain of mhcA (N-terminal 761 residues) expressed in wild typeaxeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+, mhcA(1-33/GTG/79-2116) [pM761-deltaN1], neoR
mhcA-mhcA (myosin II heavy chain) null mutantaxeA2, axeB2, axeC2, mhcA-[pΔMHC-A5], neoRaxe, neoR
mhcA-mhcA (myosin heavy chain) null mutant; this strain was not viableaxeA2, axeB2, axeC2, mhcA-[pΔMHC-A5], neoRvery slow growth; small aggregates
mhcA-mhcA (myosin heavy chain) null mutantaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY]axe,
mhcA-mhcA null mutant, neomycin resistantaxeA1, axeB1, axeC1, mhcA-[pΔMHC-A5], neoR
mhcA-mhcA-null mutant in a spontanous axenic wild type strain (identical to HK321)axe, matA1, mhcA- [pΔMHCA5], neoR
mhcA-Myosin heavy chain null mutant in NC4A2 background (strain identical to HK323)axe, matA1, mhcA- [pΔMHCA5], neoRaxe, neoR
mhcA-mhcA null mutant expressed in AX3axeA1, axeB1, axeC1, mhcA-, [bsR-loxP], bsR
mhcA-/forA-forA null mutant in mhcA nullaxeA2, axeB2, axeC2, mhcA-[pΔMHC-A5], forA-[bsR-loxP], bsR, neoR
mhcA-/piaA-piaA null mutant in mhcA nullaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], piaA-, [pKO], bsR
mhcA-/talA-talA null expressed in mhcA null strainaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], talA-, bsR
mhcA-/[act15]:GFP:abpC(9-148)N-terminal fusion of GFP to the actin binding domain of abpC (ABP-120) expressed in AX2axeA2, axeB2, axeC2, mhcA-, GFP:abpC(9-148), [pDXA-GABD], neoR
mhcA-/[act15]:GFP:ctnnActnnA (vinA) overexpressed in mhcA null cellsaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-, GFPctnnA, neoR
mhcA-/[act15]:GFP:ctxA/[act15]:enlA(92-497)expression of a truncated enlA (enlazin; residues 92-497) in GFP-ctxA labelled mhcA null cellsaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], [pREP], GFP-ctxA[pDRH-HygR][pREP], enlA(92-497)[pLD1A15SN], hygR, neoR
mhcA-/[act15]:GFP:ctxA/[RNAi]enlAexpression of a hairpin enlA construct (antisense 1-1483, sense 857-1462) in GFP-ctxA labeled mhcA null cellsaxeA1, axeB1, axeC1, pyr5-6- [PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-, [pTZD1THY], [pREP], GFP-ctxA, [pDRH-HygR][pREP], enlA(AS1-1483, S857-1462)[pLD1A15SN], hygR, neoR
mhcA-/[act15]:GFP:ctxA/[RNAi]enlAexpression of GFP-ctxA in mhcA null cells; co-transformed with pREP to provide replicase functionaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], [pREP], ctxA-GFP[pDRH-HygR][pREP], hygR
mhcA-/[act15]:GFP:mhcAN-terminal GFP-myosin fusion transformed into HS1 mhcA null cellsaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY]
mhcA-/[act15]:GFP:mhcA(E476K)N-terminal fusion of GFP to mhcA with a mutation in the motor domain rendering it ''''motor-less'''', expressed in mhcA null cellsaxeA2, axeB2, axeC2, mhcA-, GFP:mhcA(E476K), neoR
mhcA-/[act15]:GFP:mhcA/[RNAi]rmd1knockdown of rmd1 in mhcA null with GFP-tagged MhcAaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], GFP:mhcA, [pDRH], RNAi-rmd1, [pLD1A15SN], neoR
mhcA-/[act15]:GFP:pats1(2087-2351)overexpression of pats1 kinase domain (2087-2351) in an mhcA nullaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, mhcA-, ura+, [act15]:GFP:pats1(2087-2351), neoR
mhcA-/[act15]:GFP:paxBpaxB overexpressed in mhcA null cellsaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-, GFP-paxB, neoR
mhcA-/[act15]:GFP:tubA/[RNAi]enlAexpression of a hairpin enlA construct (antisense 1-1483, sense 857-1462) in mhcA null cells to achieve enlA silencing by RNAi; co-transformed with pREP to provide replicase functionaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], [pREP], GFP-tubA[pDRH-HygR], enlA(AS1-1483, S857-1462), [pLD1A15SN], neoR, hygRaxe,hygR,neoR
mhcA-/[act15]:GFP:tubA/[RNAi]enlAextrachromosomal expression of GFP-alpha tubulin in mhcA null cells; co-transformed with pREP to confer the Ddp2 ORI trans replication functionaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], [pREP], GFP-tubA[pDRH-HygR], hygRaxe,hygR
mhcA-/[act15]:gppA:GFPmhcA null cells expressing GFP-marked GppAaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], gppA:GFP, [pTX-GFP], neoR
mhcA-/[act15]:limE(1-145):GFPexpression of limE lacking the C-terminal coiled-coil domain fused at the C-terminus to GFP, in a mhcA null strain; neomycin resistantaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], limEdCoil [pDEXH-limEdCoil], neoR
mhcA-/[act15]:mhcAmhcA under control of act15 promotor in mhcA nullaxeA1, axeB1, axeC1, mhcA-, [pΔMHC-A5], mhcA, [pDE105], neoR
mhcA-/[act15]:mhcA(1-33/GTG/79-2116)Partially N-terminally truncated (residues 34 to 78 deleted) mhcA expressed in an mhcA null mutantaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], mhcA(1-33/GTG/79-2116) [deltaN2], neoR
mhcA-/[act15]:mhcA(1-785mhcA with an additional binding site for the essential myosin light chain in mhcA null axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], mhcA(1-785, RISEIIKAIQAATRGWIARKVYKQA, 786-2116), pTIKL, neoR
mhcA-/[act15]:mhcA(1-787mhcA lacking the myosin regulatory myosin light chain binding site in mhcA null axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], mhcA(1-787, 818-2116), pTIKL, neoR
mhcA-/[act15]:mhcA(1-933:1455-2116]mhcA with deletion of residues 934-1454 expressed in mhcA nullaxeA2, axeB2, axeC2, mhcA-[pΔMHC-A5], mhcA(1-933, 1455-2116], [pSB2], neoR, hygR
mhcA-/[act15]:mhcA(81-2116)N-terminally truncated (80 amino acids) mhcA expressed in an mhcA null mutantaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], mhcA(81-2116) [deltaN1], neoR
mhcA-/[act15]:mhcA(E459V)mhcA(E459V) expressed in mhcA nullaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], mhcA(E459V), [pBIG], neoR
mhcA-/[act15]:mhcA(F692A)mhcA(F692A) expressed in mhcA nullaxeA1, axeB1, axeC1, mhcA-, [pΔMHC-A5], mhcA(F692A), [pBIG], neoR
mhcA-/[act15]:mhcA(I499A)mhcA(I499A) expressed in mhcA nullaxeA1, axeB1, axeC1, mhcA-, [pΔMHC-A5], mhcA(I499A), [pBIG], neoR
mhcA-/[act15]:mhcA(S465V)mhcA(S465V) expressed in mhcA nullaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], mhcA(S465V), [pBIG], neoR
mhcA-/[act15]:mhcA(T1823A/T1833A/T2029A)cell line without antibiotic resistance marker expressing the 3xALA mutant mhcA genemhcA-, [pMHC3xALA]axe
mhcA-/[act15]:mhcA(T1823A/T1833A/T2029A)3xALA mutant mhcA, which eliminates phosphorylation at these positions, in a mhcA null backgroundaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], mhcA-ALA [pBIG-ALA], neoR
mhcA-/[act15]:mhcA(T1823D/T1833D/T2029D)3xALA mutant mhcA, which mimicks the fully phosphorylated state, in a mhcA null backgroundaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], mhcA-ASP [pBIG-ASP], neoR
mhcA-/[act15]:mhcA/[act15]:GFP:ctxAexpression of mhcA in GFP-ctxA labeled mhcA null cells; co-transformed with pREP to provide replicase functionaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], [pREP], ctxA-GFP[pDRH-HygR][pREP], mhcA[pBig], hygR, neoR
mhcA-/[act15]:rapA(G12V)myosin II (mhcA) null mutant overexpressing a constitutively active form of rapA under the control of the act15 promoteraxeA1, axeB1, axeC1, [act15/rapA(G12V)], mhcA-, neoR
mhcA-/[act15]:tubA:GFPmhcA-null cells expressing GFP-?-tubulinaxeA2, axeB2, axeC2, mhcA-[pdeltaMHC-A5], neoR, [GFP-tubulin-bsr], bsRaxe, neoR, bsR, gfp
mhcA-/[mhcA]:mhcA(1-1224)Insertion of the mhcA N-terminal 1224 amino acids (HMM) in the promoter region of the endogenous mhcA gene, disrupting the endogenous gene and expressing the fragmentaxeA1, axeB1, axeC1, mhcA-, hmm [pA15TX-HMM-140], neoR
mhcA-/[mhcA]:mhcA(1-1224)/[act15]:mhcA(E683K)expression of a E683K point mutation in the S1 motor domain of mhcA in an effectively mhcA disrupted strain axeA1, axeB1, axeC1, mhcA-, [mhcA]:mhcA(1-1224), [pA15TX-HMM-140], [act15]:mhcA(E683K), [pTIKLMyDAPE683K], neoR
mhcA-/[mhcA]:mhcA(1-1224)/[act15]:mhcA(R686C)expression of a R686C point mutation in the S1 motor domain of mhcA in an effectively mhcA disrupted strain axeA1, axeB1, axeC1, mhcA-, [mhcA]:mhcA(1-1224), [pA15TX-HMM-140], [act15]:mhcA(R686C), [pTIKLMyDAPR686C], neoR
mhcA-/[mhcA]:mhcA(1-1224)/[act15]:mhcA(R689H)expression of a R689H mutation in the S1 motor domain of mhcA in an effectively mhcA disrupted strainaxeA1, axeB1, axeC1, mhcA-, hmm [pA15TX-HMM-140], mhcAR689H [pTIKLMyDAPR689H], neoR
mhcA-/[unk]:GFP:icpAGFP-icpA expressed in an mhcA null mutantmhcA-, [GFP-icpA]
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