| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| [unk]:GFP:wasA(I173A/F179A) | GFP-marked wasA(I173A/F179A) in AX3 | axeA1, axeB1, axeC1, wasA(I173A/F179A) | |
| [unk]:gpgA(1-65) | gpgA(1-65) in AX2 | axeA2, axeB2, axeC2, gpgA(1-65), [p88d1], neoR | |
| [unk]:mhcA(T1823A/T1833A/T2029A) | triple T to A mutant of myosin heavy chain expressed in wild type | axeA2, axeB2, axeC2, [myo3xTA], neoR? | axe,neoR? |
| [unk]:mhkC(1-498) | mhkC lacking the C-terminal WD repeat domain in AX2 | axeA2, axeB2, axeC2, mhkC(1-498), [pGEX-2T], neoR | |
| [unk]:myc:rgaA(K273E/K282E) | RgaA with K273E, K282E substitutions expressed in KAX3 cells. | axeA1, axeB1, axeC1, rgaA(K273E/K282E) | |
| [unk]:pdsA | psdA overexpressed in AX3 | axeA1, axeB1, axeC1, pdsA, [pB10TP1], neoR | |
| [unk]:pikA(1-492(S7A/S11A/S109A/S113A/S177A/S181A)) | PikA membrane localization domain containing serine to alanine substitutions at 6 potential phosphorylation sites expressed in JH10 background; phosphomimetic mutant | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-, pikA(1-492(S7A/S11A/S109A/S113A/S177A/S181A)), [pEXP4(+)], neoR | |
| [unk]:pikI(T262E) | phosphomimetic PikI with T262E substitution in AX2 | axeA2, axeB2, axeC2, pikI(T262E) | |
| [unk]:pkgB(T309A)/pkgB- | pkbR1- expressing pkbR1(T309A), catalytically inactive; complements the developmental phenotype of pkgB- | axeA1, axeB1, axeC1, pkgB-, pkgB(T309A), bsR | |
| [unk]:pkgB(T470A)/pkgB- | pkbR1- expressing pkbR1(T470A), catalytically inactive; complements the developmental phenotype of pkgB- | axeA1, axeB1, axeC1, pkgB-, pkgB(T470A), bsR | |
| [unk]:PTEN(H385G/N389A/H390A) | pten containing neutral amino acid substitutions for basic amino acids in the c-alpha-2 helix region, expressed in AX2 cells | axeA2, axeB2, axeC2, pten((H385G/N389A/H390A), neoR | |
| [unk]:rapA(G12V) | constitutively active rapA expressed in AX3 cells | axeA1, axeB1, axeC1, rapA(G12V), neoR | |
| [unk]:rasD(G12T) | overexpressor of rasD(G12T) | axeA1, axeB1, axeC1, rasD(G12T), neoR | |
| [unk]:rasD(G12T)/[rasD]:rapA | RapA expressed under control of rasD promotor expressed in rasD(G12T) | axeA1, axeB1, axeC1, rasD(G12T), [rasD]:rapA, [pRasDNeoRap1], neoR, hygR | |
| [unk]:rasD:GFP | overexpressor of rasD-GFP in AX2; neomycin resistant | axeA2, axeB2, axeC2, [rasD], neoR | |
| [unk]:rasG(Q61L)/rasG- | rasG null expressing a constitutively active RasG(Q61L) | axe, rasG-, rasG(Q61L) | |
| [unk]:roco4(1018-1292(G1179S)) | Roco4 kinase domain with glycine to serine substitution at position 1179 in AX2 | axeA2, axeB2, axeC2, roco4(1018-1292(G1179S)), [pGEX4T1], bsR | |
| [unk]:roco4(1018-1292;F1107L | Roco4(1018-1292) with substitutions F1107L and F1161L to resemble the active site of human LRRK2 | axeA2, axeB2, axeC2, roco4(1018-1292;F1107L, F1161L), (pGEX4T1) | |
| [unk]:scrA(97-443):GFP | wild type cells expressing scrA with a deletion of the first 96 amino acids and fused to GFP | axeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, src(97-443):GFP, neoR | |
| [unk]:scrA(97-443):GFP | wild type cells expressing scrA with a deletion of the first 96 amino acids and fused to GFP | axeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, src(97-443):GFP, neoR | |
| [unk]:tps9(N236A) | tps9 with (N236A) substitution in AX4 | axeA1, axeB1, axeC1, tps9(N236A), [pGEM-TEasy] | |
| [unk]:ttpA(1-236) | ttpA(1-236) truncated by a frameshift after E 236 in AX3 | axeA1, axeB1, axeC1, ttpA(1-236), [pTM1285] | |
| [unk]:ttpA(1-242) | ttpA(1-242) truncated by a frameshift after Y 242 in AX3 | axeA1, axeB1, axeC1, ttpA(1-242), [pTM1285] | |
| [unk]:ttpA(1-450) | ttpA(1-450) in AX3 | axeA1, axeB1, axeC1, ttpA(1-450), [pTM1285] | |
| [unk]:tubA:GFP/RNAi]cepG | knockdown of the cepG gene using RNAi in GFP-alpha-tubulin strain | axeA2, axeB2, axeC2, GFP:tubA, [RNAi]cepG, [pIS193], bsR, neoR | |
| [unk]:tubA:GFP/[RNAi]cepD | knockdown of the cepD gene using RNAi in GFP-alpha-tubulin strain | axeA2, axeB2, axeC2, GFP:tubA, [RNAi]cepD, [pIS193], bsR, neoR | |
| [unk]:tubA:GFP/[RNAi]cepL | knockdown of the cepL gene using RNAi in GFP-alpha-tubulin strain | axeA2, axeB2, axeC2, GFP:tubA, [RNAi]cepL, [pIS1109], bsR, neoR | |
| [unk]:vacA:myc:vasP | hybrid of vacA fused to vasP with myc linker expressed in AX2 | axeA2, axeB2, axeC2, vacA:myc:vasP, [pGEM-Teasy] | |
| [unk]:vasP(1-197:221-261:285-380) | Vasp lacking the G-actin binding domain and the F-actin binding domain expressed in AX2 cells | axeA2, axeB2, axeC2, vasP(1-197:221-261:285-380), neoR | |
| [unk]:vasP(1-197:221-380) | Vasp lacking the G-actin binding domain expressed in AX2 cells | axeA2, axeB2, axeC2, vasP(1-197;221-380), neoR | |
| [unk]:vasP(1-261:285-380) | Vasp lacking the F-actin binding domain expressed in AX2 cells | axeA2, axeB2, axeC2, vasP(1-261:285-380), neoR | |
| [unk]:vasP(1-340:376-380) | Vasp lacking the tetramerization domain expressed in AX2 cells | axeA2, axeB2, axeC2, vasP(1-340:376-380), neoR | |
| [unk]skp1 | skp1 (fpaA/fpaB) overexpressed under the control of an unknown promoter | axeA1, axeB1, axeC1, skp1 | |
| [unk]wasA:GFP | wild type HPS400 cells expressing wasA (WASP)-GFP | axeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, wasA:GFP, neoR | |
| [unsuccessful]adprt2/adprt1B- | attempts to produce a adprt1B-/adprt2 double null mutant failed in an AX2 background | axeA2, axeB2, axeC2, adprt2-[[bsR-loxP], bsR | |
| [unsuccessful]aurK- | attempts to produce an aurK null mutant failed, as expected for a protein participating in multiple steps of mitosis | axeA2, axeB2, axeC2 | |
| [unsuccessful]cak1- | attempts to produce a cak1 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]cca1- | attempts to produce a cca1 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]cca2- | attempts to produce a cca2 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]cdk5- | attempts to produce a cdk5 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]cepA- | attempts to produce a cepA null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]cepD- | attempts to produce a cepD null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]cepL- | attempts to produce a cepL null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]cepL- | attempts to produce a cepL null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]cinC- | attempts to produce a cinC null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]csn2- | attempts to produce a csn2 null mutant failed with several different constructs | axeA1, axeB1, axeC1 | |
| [unsuccessful]csn5- | attempts to produce a csn5 null mutant failed with several different constructs | axeA1, axeB1, axeC1 | |
| [unsuccessful]DDB_G0275923- | attempts to produce a DDB_G0275923 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]DDB_G0285927- | attempts to produce a DDB_G0285927 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]dhcA- | attempts to produce a dhcA null mutant failed in both AX2 and JH-10 cells | axeA2, axeB2, axeC2 | |
| [unsuccessful]dhcA- | attempts to produce a dhcA antisense mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]dhx9- | attempts to produce a dhx9 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]dicA- | attempts to produce a dicA null mutant failed | axeA1, axeB1, axeC1 | |
| [unsuccessful]drnA- | attempts to produce a drnA null mutant failed
| axeA2, axeB2, axeC2 | |
| [unsuccessful]eIF6- | attempts to produce a eIF6 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]fr1B | attempts to produce a fr1B null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]fttB- | numerous attempts to disrupt the fttB gene were unsuccessful | axeA2, axeB2, axeC2 | |
| [unsuccessful]gefF- | attempts to produce a gefF- null mutant failed | axeA1, axeB1, axeC1 | |
| [unsuccessful]gpbB- | attempts to produce a gpbB null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]H3a | attempts to produce a H3a null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]hcpA-/hcpB- | attempts to produce a hcpA/hcpB double null mutant failed, starting from a hcpA null in an AX2 background | axeA2, axeB2, axeC2, hcpA-[hcpA-bsr], bsR | |
| [unsuccessful]hdaA- | attempts to produce a hdaA null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]hsp101- | attempts to produce a hsp101 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]ifkB- | attempts to produce a ifkB null mutant failed with several different constructs | axeA1, axeB1, axeC1 | |
| [unsuccessful]kif2 | kif2 could not be overexpressed; no transformant was obtained after antibiotic selection, suggesting that a high level of exogenous expression of K2 under control of the AX2 promotor was lethal | axeA2, axeB2, axeC2 | |
| [unsuccessful]kif3- | attempts to produce a kif3 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]kif6- | attempts to produce a kif6 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]lis1- | attempts to produce a lis1 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]mppA1- | attempts to produce a mppA1- null mutant failed | axeA1, axeB1, axeC1 | |
| [unsuccessful]mppB- | attempts to produce a mppB- null mutant failed; seems to be essential like in S. cerevisiae and C. elegans | axeA1, axeB1, axeC1 | |
| [unsuccessful]nkaP- | attempts to produce a nkaP null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]nola4- | attempts to produce a nola4 null mutant failed | axeA1, axeB1, axeC1 | |
| [unsuccessful]padA- | attempts to produce a padA- null mutant failed repeatedly | axeA2, axeB2, axeC2 | |
| [unsuccessful]pho2A- | attempts to produce a pho2A- null mutant failed repeatedly | axe, matA1 | |
| [unsuccessful]pho2B- | attempts to produce a pho2B null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]phr2AB- | attempts to produce a phr2AB- null mutant failed repeatedly | axe, matA1 | |
| [unsuccessful]pikA-/pikC- | attempts to produce a pikA-/pikC- double null mutant failed either starting from pikA null or from pikC null | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, pikA-[k1T-3], thy+ | |
| [unsuccessful]pikB-/pikC- | attempts to produce a pikB-/pikC- double null mutant failed either starting from pikB null or from pikC null | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, pikB-[K2D-2], thy+ | |
| [unsuccessful]pikD- | attempts to produce a pikD- null mutant failed | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy- | |
| [unsuccessful]pppA- | attempts to produce a pppA null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]pppB- | attempts to produce a pppB null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]ptpC- | attempts to produce a ptpC- null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]rab1A- | attempts to produce a rab1A null mutant failed | axeA1, axeB1, axeC1 | |
| [unsuccessful]rab8a- | attempts to produce a rab8A null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]rapA- | attempts to produce a rapA null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]repB(1-705) | attempts to produce a repB mutant with the last 95 aa deleted failed | axeA1, axeB1, axeC1 | |
| [unsuccessful]rpb4- | attempts to produce a rpb4- null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]rps6- | attempts to produce a rps6 null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]sbdS- | attempts to produce a sbdS null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]sibA-/sibC- | attempts to produce a double knock-out failed, sibA-/sibC- double null mutant failed | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura- | |
| [unsuccessful]sir2E- | attempts to produce a sir2E- null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]sodA- | attempts to produce a sodA- null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]sodB- | attempts to produce a sodB- null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]srtA- | attempts to produce an srtA null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]sun1- | attempts to produce a sun1- null mutant failed as well as extreme down-regulation | axeA2, axeB2, axeC2 | |
| [unsuccessful]tcp1- | attempts to produce a tcp1- null mutant failed | axeA1, axeB1, axeC1 | |
| [unsuccessful]tf2 | attempts to produce a tf2 null mutant by homologous recombination and bsR selection failed | axeA1, axeB1, axeC1 | |
| [unsuccessful]top2mt- | attempts to produce a top2mt- (topA-) null mutant failed | axeA1, axeB1, axeC1 | |
| [unsuccessful]tor- | attempts to produce a tor null mutant failed | axeA2, axeB2, axeC2 | |
| [unsuccessful]trrA- | attempts to produce a trrA- null mutant failed as well as knock-down by constitutive antisense-mediated inhibition | axeA1, axeB1, axeC1 | |
