| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| [act6]:ptpC(C649S) | overexpressor of ptpC(C649S) (catalytic dead) under the control of the act6 promoter | axeA1, axeB1, axeC1, [act6]:ptpC(C649S), neoR | |
| [act6]:rabS(N137I):GFP | C-terminal GFP-tagged rabS with N137I substitution in AX4 | axeA1, axeB1, axeC1, rabS(N137I):GFP, [pDneo2-GFP], neoR | |
| [act6]:rabS:GFP | C-terminal GFP-tagged rabS in AX4 | axeA1, axeB1, axeC1, rabS:GFP, [pDneo2-GFP], neoR | |
| [act6]:rps6 | rps6 under control of act6 promotor overexpressed in AX2 | axeA2, axeB2, axeC2, rps6, [pDNeo67], neoR | |
| [act6]:srsA | srsA overexpressed in wild type | axeA2, axeB2, axeC2, srsA, neoR | |
| [act6]:trap1 | overexpressor of Dd-TRAP1 (TRAP-1 homolog); neomycin resistant | axeA2, axeB2, axeC2, [pDNeo2-Dd-TRAP1], neoR | |
| [act6]:ubxd9(1-336) | ubxd9(1-336) expressed in AX2 under control of the act6 promotor | axeA2, axeB2, axeC2, ubxd9(1-336), [pPB86], bsR | |
| [act6]:ubxd9(165-383) | ubxd9(165-383) expressed in AX2 under control of the act6 promotor | axeA2, axeB2, axeC2, ubx9(165-383), [pPB86], bsR | |
| [act6]:ubxd9(467-573) | ubxd9(467-573) expressed in AX2 under control of the act6 promotor | axeA2, axeB2, axeC2, ubxd9(467-573), [pPB86], bsR | |
| [act6]:yakA | YakA under the control of the act6 promotor in AX4 cells | axeA1, axeB1, axeC1, yakA, [pDNeo67], neoR | |
| [act6]:zizB:GFP | GFP marked ZizB over-expressed in AX2 cells | axeA2, axeB2, axeC2, [act6]:zizA:GFP, hygR | |
| [act]:alrA/gcsA- | alrA overexpressor in gcsA null mutant | axeA1, axeB1, axeC1, gcsA-, act:alrA, [pGEX-4T], bsR, neoR | |
| [ampA]:RFP:ampA | ampA tagged with N-terminal RFP in AX3; this plasmid was electroporated as a closed plasmid and has an over expressor phenotype | axeA1, axeB1, axeC1, [ampA]:RFP/ampA, bsr | |
| [arkA]:arkA:myc2 | double myc-tagged arkA overexpressed from its own promoter; neomycin resistant | axeA1, axeB1, axeC1, [arkA]:arkA:myc2, neoR | |
| [arpB]:arpB:GFP | arpB-GFP replaces endogenous arpB gene, resulting in an impairment of the ARP2/3 complex. (Knock-in of enhanced GFP to the C-terminus of arpB with a TRGAG linker). | axeA2, axeB2, axeC2, arpB[Arp2-GFP], bsR | axe, bsR |
| [AS]:ndkC | ndkC antisense mutant | axeA2, axeB2, axeC2, [AS]:ndkC, neoR | |
| [AS]:[rpl11]:lmcB | lmcB antisense mutant under control of the V18 (rpl11) promotor | axeA1, axeB1, axeC1, [AS]:lmcB, neoR | |
| [AS]:[tet]:trrA | trrA antisense under the control of the tetracycline promoter | axeA1, axeB1, axeC1, [AS]trrA, bsR, neoR | |
| [AS]act15:adprt1A | adprt1A antisense mutant under the control of the act15 promoter; GFP in the vector was replaced with AS sequence; down regulated about 60% | axeA2, axeB2, axeC2, adprt1A-AS[pTX-PARP];neoR | |
| [AS]aif | aif antisense mutant | axeA2, axeB2, axeC2, [AS]aif, [pTX-GFP], neoR | |
| [AS]alrA/gcsA- | alrA antisense in gcsA null mutant | axeA1, axeB1, axeC1, gcsA-, bsR, [act15/AS:alrA], neoR | |
| [AS]calA | calA antisense under a discoidin-inducible promoter | axeA2, axeB2, axeC2, [AS]calA | |
| [AS]carA | carA antisense mutant | axeA1, axeB1, axeC1, [AS]carA, neoR | |
| [AS]cf60 | partial knockdown of the cf60 gene using antisense; neomycin resistant | axeA2, axeB2, axeC2, [cf60as], neoR | |
| [AS]cfrA/[AS]cfrB | GP138 antisense; inhibits GP138A and GP138B due to high sequence similarity | axeA1, axeB1, axeC1, [AS]cfrA, [AS]cfrB | |
| [AS]chcA | chcA antisense mutant | axeA2, axeB2, axeC2, chcA-AS, neoR | |
| [AS]cmfA | cmfA antisense mutant | axeA1, axeB1, axeC1, [pDNeo2-cmfA-AS], neoR | |
| [AS]csbA/csbB | csbA /csbB tandem antisense | axeA1, axeB1, axeC1, [pDdNeoII-csbA/csbB-as], neoR | |
| [AS]cupB | cupB antisense; neomycin resistant | axeA2, axeB2, axeC2, [cupB-AS], neoR | |
| [AS]deeJ | deeJ (DJ-1) antisense mutant | axeA2, axeB2, axeC2, deeJ, [pPROF688], neoR | |
| [AS]deeJ/[AS]snfA | deeJ/snfA double antisense mutant by co-transformation in AX2 | axeA2, axeB2, axeC2, [AS]deeJ, [pPROF688], [AS]snfA, [pPROF362], neoR | |
| [AS]dia1 | dia1 antisense mutant | axeA2, axeB2, axeC2, neoR, [act15]:[AS]dia1 | |
| [AS]dscA | dscA antisense mutant that also represses expression of dscC and dscD | axeA1, axeB1, axeC1, [AS]dscA, pB10S, neoR | |
| [AS]dutA | dutA antisense mutant | axeA2, axeB2, axeC2, neoR, dutA- | |
| [AS]ecmB:adprt1A | adprt1A antisense mutant under control of the ecmB promoter; LacZ in the vector was replaced with AS sequence; down regulated about 60% | axeA2, axeB2, axeC2, adprt1A-AS[pEcmB-PARP];neoR | |
| [AS]erkA | erkA antisense mutant with an inducible discoidin promotor | axeA1, axeB1, axeC1, erkA, [pVEII], neoR | |
| [AS]gpaA | gpaA antisense mutant | axeA1, axeB1, axeC1, [gpaA]:[AS]gpaA, neoR | |
| [AS]gshB | gshB antisense mutant | axeA1, axeB1, axeC1, gshB-, [pTX-FLAG], neoR | |
| [AS]hspA | hspA (chaperonin 60) antisense mutant | axeA2, axeB2, axeC2, [pPROF128], neoR | axe,neoR |
| [AS]hspA/snfA | hspA/snfA double antisense mutant tranformed by cotransformation | axeA2, axeB2, axeC2, [pPROF362/pPROF128], neoR | |
| [AS]hspA/[AS]ndkC-1 | ndkC antisense mutant coexpressed with hspA antisense mutant in AX2 | axeA2, axeB2, axeC2, [pPROF128], [pPROF500], neoR | |
| [AS]hssA | hssA antisense mutant | axeA2, axeB2, axeC2, [AS]hssA- | |
| [AS]htrA | htrA antisense mutant | axeA2, axeB2, axeC2, [AS]htrA, [pDNeo2], neoR | |
| [AS]ino1 | ino1 antisense under the control of the act15 promoter; inositol auxotroph | axeA2, axeB2, axeC2, [pDEX-INO1], neoR | |
| [AS]mcln | mcln antisense mutant | axeA2, axeB2, axeC2, mcln, [pDNeo2], neoR | |
| [AS]mppA1 | mppA antisense mutant | axeA1, axeB1, axeC1, AS-mppA [pDNeo 67], neoR | |
| [AS]mppB | mppB antisense mutant | axeA1, axeB1, axeC1, AmppB [pDNeo 67], neoR | |
| [AS]PARP | down-regulation of all PARP (poly(ADP-ribose) polymerase) genes by antisense, namely adprt1A, adprt1B, adprt2, adprt3, adprt4, pARTf, pARTg, DDB_G0286613, DDB_G0289141 | axeA2, axeB2, axeC2, adprt1A, adprt1B, adprt2, adprt3, adprt4, pARTf, pARTg, DDB_G0286613, DDB_G0289141-AS[pTX-PARP, neoR | |
| [AS]patA | patA antisense mutant | axeA2, axeB2, axeC2, [EXPpatA-AS], neoR | axe,neoR,poor growth in high calcium medium |
| [AS]pikE | pikE antisense mutant | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-, neoR, [AS]pikE | |
| [AS]ppiA | ppiA antisense | axeA1, axeB1, axeC1, [AS]ppiA, neoR | |
| [AS]proA | Profilin I antisense strain | axeA2, axeB2, axeC2, [pDexRH-pI-ascDNA], neoR | |
| [AS]proA-/proB-/lmpA- | lmpA null cells expressed in profilin double null mutant | axeA2, axeB2, axeC2, proA-[pDexRH-pI-ascDNA], proB-[pDexRH-pII-cDNA], lmpA-, [pUCBsrΔBam], bsR, neoR | |
| [AS]proA/abpB(129-295) | abpB residues 129-295 expressed in proA antisense strain | axeA2, axeB2, axeC2, [pDexRH-pI-ascDNA], abpB(129-295), [pBORP], neoR | |
| [AS]proA/proB- | proA and proB double knockout (proB is deletion, proA is antisense) | axeA2, axeB2, axeC2, proA-[pDexRH-pI-ascDNA], neoR, proB-[pDexRH-pII-cDNA], FLAG:proC, ? | |
| [AS]proA/proB-/scrA- | scrA null mutant expressed in proA/proB double null mutant | axeA2, axeB2, axeC2, proA-[pDexRH-pI-ascDNA], proB-[pDexRH-pII-cDNA], scrA-[9A/O7C11], bsR, neoR | |
| [AS]proA/proB-/[act15]:proA | proA and proB double mutant rescued by proA expression | axeA2, axeB2, axeC2, proA-[pDexRH-pI-ascDNA], neoR, proB-[pDexRH-pII-cDNA], proA, hygR | |
| [AS]proA/proB-/[act15]:proB | proA/proB double mutant rescued by proB expression | axeA2, axeB2, axeC2, proA-[pDexRH-pI-ascDNA], neoR, proB-[pDexRH-pII-cDNA], proB, hygR | |
| [AS]proA/proB-/[unk]:FLAG:proC | proA/B double mutant overexpressing FLAG-tagged proC; rescues double mutant phenotype | axeA2, axeB2, axeC2, proA-[pDexRH-pI-ascDNA], neoR, proB-[pDexRH-pII-cDNA], FLAG:proC, ? | |
| [AS]ptcB | ptcB antisense mutant | axeA2, axeB2, axeC2, [pDNeo2-ptcB-AS], neoR | |
| [AS]rapA | rapA antisense mutant | axeA2, axeB2, axeC2, neoR, rapA-AS | |
| [AS]rheb | rheb antisense mutant | axeA2, axeB2, axeC2, [AS]rheb, [pDneo2], neoR | |
| [AS]rps4 | rps4 antisense mutant in AX2 | axeA2, axeB2, axeC2, rps4, [pACT15XPT1], neoR | |
| [AS]rps4 | rps4 antisense cells (mitochondrial ribosomal protein S4) | axeA2, axeB2, axeC2, [rps4-AS vector], neoR | axe,neoR |
| [AS]rtoA | rtoA antisense | axeA1, axeB1, axeC1, [AS]rtoA, neoR | |
| [AS]sdhA | sdhA antisense mutant | axeA2, axeB2, axeC2, sdhA, [pPROF227], neoR | |
| [AS]sdhB | sdhB antisense mutant | axeA2, axeB2, axeC2, sdhB, [pPROF227], neoR | |
| [AS]sdhC | sdhC antisense mutant | axeA2, axeB2, axeC2, sdhC, [pPROF227], neoR | |
| [AS]smlA | smlA antisense | axeA1, axeB1, axeC1, [AS]smlA, neoR | axe, neoR |
| [AS]snfA | snfA (AMPKalpha) antisense mutant | axeA2, axeB2, axeC2, [pPROF362], neoR | |
| [AS]snfA/[AS]sdhA | snfA/sdhA double antisense mutant in AX2 | axeA2, axeB2, axeC2, [AS]snfA, [pPROF362], sdhA, [pPROF227], neoR | |
| [AS]sslA1 | sslA1 antisense mutant | axeA1, axeB1, axeC1, As-sslA1 | |
| [AS]tcp1 | tcp1 antisense mutant | axeA1, axeB1, axeC1, [pAG03as]; neoR | |
| [AS]tpp1 | tpp1 antisense mutant | axeA2, axeB2, axeC2, [AS]tpp1, [pDneo2], neoR | |
| [AS]twm1 | twm1 antisense mutant | axeA2, axeB2, axeC2, [AS]twm1, [pDNeo2], neoR | |
| [AS]vatB | vatB antisense under a discoidin-inducible promoter | axeA2, axeB2, axeC2, vatB, [pVEII], neoR | |
| [AS]vatP | vatP antisense mutant; integrative vector (pVEII-AS) carrying an inducible discoidin promoter; after a few weeks mutants either die or revert. | axea2, axeB2, axeC2, vatP[pVEII-AS], neoR | |
| [AS]vatP | vatP antisense mutant; extrachromosomal vector (pRHI9-AS) possessing the constitutive act15 promoter; after a few weeks mutants either die or revert. | axea2, axeB2, axeC2, vatP[pRHI9-AS], neoR | |
| [carA]:carA(1-317) | cAR1 partial deletion in AX3; deleted C-terminal end after residue 317 | axeA1, axeB1, axeC1, carA-[Bluescript-NPT1], neoR | axe,neoR,sparse fruiting |
| [cotB]:FLAG:fbxD | FLAG tagged fbxD expressed in AX3 under control of the prespore specific cotB promoter | axeA1, axeB1, axeC1[cotB]:FLAG:fbxD, [pVSC], neoR | |
| [cotB]:fpaB:myc | fpaB expressed under the control of the prespore cotB promoter and a C-terminal Myc fusion; 4-5 times overexpressed | axeA1, axeB1, axeC1, [cotB]:fpaB:myc, neoR | |
| [cotB]:GFP | AX4 cells expressing GFP under the control of the prespore cotB promoter | axeA1, axeB1, axeC1, [cotB::GFP], neoR | axe,neoR,wt |
| [cotB]:lacZ | HL328 cells expressing lacZ under the control of the prespore cotB promoter; uracil auxotroph | axeA1, axeB1, axeC1, pyr5-6-[pdeltaPYR-BglII], ura-, [cotB::lacZ], neoR | |
| [cotB]:lacZ | AX4 cells expressing lacZ under the control of the prespore cotB promoter | axeA1, axeB1, axeC1, [cotB::lacZ], neoR | |
| [cotC]:carB | HPS400 expressing carB under the control of the prespore cotC (sp60) promoter | axeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, thy-, [cotC:carB], neoR | axe, neoR, cycR, acrR, ebR, thy-, tsg |
| [cotC]:pkaC | pkaC overexpressed under control of the prespore cotC promotor in AX3 cells | axeA1, axeB1, axeC1, pkaC, neoR | |
| [cotC]:pkaC/[spiA]:lacZ | lacZ expressed by spiA promotor in cells overexpressing pkaC from the prespore cotC promotor | axeA1, axeB1, axeC1, pkaC, lacZ, neoR | |
| [cpnA]:lacZ | NC4A2 cells expressing LacZ under the control of the cpnA promotor | axe, matA1, [cpnA/lacZ], neoR | |
| [cycC]:cycC:TAP | C-terminal TAP-tagged CycC overexpressed in AX2 under control of the cycC promotor | axeA2, axeB2, axeC2, [cycC:cycC:TAP], neoR | |
| [dhkK]:GFP | AX2 cells expressing GFP under the control of a 1.6 kb dhkK promoter fragment | axeA2, axeB2, axeC2, [dhkK/GFP] | |
| [dhkK]:lacZ | AX2 cells expressing GFP under the control of a 1.6 kb dhkK promoter fragment | axeA2, axeB2, axeC2, [dhkK/lacZ] | |
| [disc]:HA:rab14(N121I) | inducible dominant negative rab14 mutant; removal of folate from the medium induces transcription of the dom/neg rab14 from the discoidin promoter | axeA1, axeB1, axeC1, [disc]:HA:rab14(N121I), neoR | |
| [disc]:HA:rab14(Q67L) | inducible constitutively active rab14 mutant; removal of folate from the medium induces transcription of the active rab14 from the discoidin promoter | axeA1, axeB1, axeC1, [disc]:HA:rab14(Q67L), neoR | |
| [dscA]:His:agtA | His-tagged overexpression of agtA in Ax3 | axeA1, axeB1, axeC1, dscA:agtA, neoR | |
| [dscA]:His:agtA(D132A) | catalytically dead AgtA over expressed under the discoidin promoter in AX3 | axeA1, axeB1, axeC1, dscA:agtA(D132A), neoR | |
| [dscB]:abpB(E60K):GFP | GFP-tagged AbpB with E60K substitution expressed in AX2 cells under control of the dscB promotor | axeA2, axeB2, axeC2, abpB(E60K):GFP, [pVEII], neoR | |
| [dscB]:cafA | folate-inducible, cafA under the control of the dscB promoter in VI88 cells | axeA2, axeB2, axeC2, cafA, [pVEII], neoR | |
| [dscB]:cycB(106-436) | overexpressor of cycB missing the destruction box | axeA2, axeB2, axeC2, [dscB]:cycB(106-436), neoR | |
| [dscB]:dicA(1-278) | N-terminal portion of dicA (1-278) under control of folate-inducible dscB promotor in AX3 | axeA1, axeB1, axeC1, dicA(1-278), [pVEII], neoR | |
| [dscB]:dicA(48-654) | C-terminal portion of dicA (48-654) under control of folate-inducible dscB promotor in AX3 | axeA1, axeB1, axeC1, dicA(48-654), [pVEII], neoR | |
