| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| [dscB]:GFP:rab8A | overexpressor of rab8A tagged with GFP at the amino terminus | axeA2, axeB2, axeC2, [dscB]:GFP:ras8A, neoR | |
| [dscB]:GFP:rab8A(N128I) | overexpressor of rab8A(N128I)tagged with GFP at the amino terminus | axeA2, axeB2, axeC2, [dscB]:GFP:ras8A(N128I), neoR | |
| [dscB]:GFP:rab8A(Q74L) | overexpressor of rab8A(Q74L) tagged with GFP at the amino terminus | axeA2, axeB2, axeC2, [dscB]:GFP:ras8A(Q74L), neoR | |
| [dscB]:GFP:rab8B | overexpressor of rab8B | axeA2, axeB2, axeC2, [dscB]:GFP:ras8B, neoR | |
| [dscB]:GFP:rab8B(Q74L) | overexpressor of rab8B(Q74L) tagged with GFP at the amino terminus | axeA2, axeB2, axeC2, [dscB]:GFP:ras8B(Q74L), neoR | |
| [dscB]:GFP:vamp7A | GFP-VAMP7 expressed in DH1-10 cells | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, GFP:VAMP7, neoR | |
| [dscB]:GFP:vamp7A(L35A) | GFP-VAMP7[L35A] mutant (dileucine signal ablated) expressed in DH-10 cells | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, GFP:VAMP7(L35A), neoR | |
| [dscB]:HA:racC | folate-inducible, racC under the control of the dscB promoter in AX2 | axeA2, axeB2, axeC2, racC, [pVEII], neoR | |
| [dscB]:HA:rapB(G12V) | folate-inducible, N-terminal HA-tagged, constitutively active RapB with G12V substitution under the control of the dscB promoter in AX4 cells | axeA1, axeB1, axeC1, HA:rapA(G12V), [pVEII], neoR | |
| [dscB]:myc:rac1B | overexpressor of myc-tagged rac1B | axeA1, axeB1, axeC1, [dscB]:myc:rac1B, neoR | |
| [dscB]:myc:rac1B(G12V) | overexpressor of myc-tagged rac1B(G12V) (constitutively active) | axeA1, axeB1, axeC1, [dscB]:myc:rac1B(G12V), neoR | |
| [dscB]:myc:rac1B(Q61L) | overexpressor of myc-tagged rac1B(Q61L) (constitutively active) | axeA1, axeB1, axeC1, [dscB]:myc:rac1B(Q61L) neoR | |
| [dscB]:myc:rac1B(T17N) | overexpressor of myc-tagged rac1B(T17N) (constitutively inctive) | axeA1, axeB1, axeC1, [dscB]:myc:rac1B(T17N) neoR | |
| [dscB]:rac1B(G12V):myc | folate-inducible, constitutively active Rac1B with G12V substitution under the control of the dscB promoter in AX3-ORF+ | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+, rac1B(G12V), [pDXD-3C], neoR | |
| [dscB]:rac1B(Q61L):myc | folate-inducible, constitutively active Rac1B with Q61L substitution under the control of the dscB promoter in AX3-ORF+ | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+, rac1B(Q61L), [pDXD-3C], neoR | |
| [dscB]:rac1B(T17N):myc | folate-inducible, dominant negative Rac1B with T17N substitution under the control of the dscB promoter in AX3-ORF+ | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+, rac1B(T17N), [pDXD-3C], neoR | |
| [dscB]:rapA | folate-inducible, RapA under the control of the dscB promoter in AX2 cells | axeA2, axeB2, axeC2, rapA, [pVEII], neoR | |
| [dscB]:rapA(D40E) | folate-inducible, RapA with D40E substitution under the control of the dscB promoter in AX2 cells | axeA2, axeB2, axeC2, rapA(D40E), [pVEII], neoR | |
| [dscB]:rapA(F160L) | folate-inducible, RapA with F160L substitution under the control of the dscB promoter in AX2 cells | axeA2, axeB2, axeC2, rapA(F160L), [pVEII], neoR | |
| [dscB]:rapA(G12T) | folate-inducible, constitutively active RapA with G12T substitution under the control of the dscB promoter in AX2 cell | axeA2, axeB2, axeC2, rapA(G12T), [pVEII], neoR | |
| [dscB]:rapA(G12V) | rapA(G12V) under the control of the discoidin promoter | axeA2, axeB2, axeC2, [dscB]:rapA(G12V), neoR | |
| [dscB]:rapA(S19N) | folate-inducible, RapA with S19N substitution under the control of the dscB promoter in AX2 cells | axeA2, axeB2, axeC2, rapA(S19N), [pVEII], neoR | |
| [dscB]:rapA(T63Q) | folate-inducible, RapA with T63Q substitution under the control of the dscB promoter in AX2 cells | axeA2, axeB2, axeC2, rapA(T63Q), [pVEII], neoR | |
| [dscB]:rasG(G12T) | rasG(G12T) under the control of the discoidin promoter (constitutively active) | axeA2, axeB2, axeC2, [dscB]:rasG(G12T), neoR | |
| [dscB]:rasG(G12T/T45Q) | constitutively active rasG(G12T/T45Q under the control of the discoidin promoter | axeA2, axeB2, axeC2, [dscB]:rasG(G12T/T45Q), neoR | |
| [dscB]:rasG(S17N) | rasG(S17N) under the control of the discoidin promoter (dominant negative) | axeA2, axeB2, axeC2, [dscB]:rasG(S17N), neoR | |
| [dscB]:RFP:syn7A | RFP-SYN7A under the control of the discoidin promoter, expressed in DH1-10 cells | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, GFP:syn7A, bsR | |
| [dscC]:kif2 | kif2 under control of dscC promotor overexpressed in AX2 | axeA2, axeB2, axeC2, kif2, [pBIG], neoR | |
| [ecmAO]:ile-GFP | unstable GFP which does not accumulate with time and thus gives generally weak
fluorescence | axeA2, axeB2, axeC2, [ecmAO/ile-wt-GFP]. neoR | neoR |
| [ecmAO]:rasG(G12T) | constitutively active rasG with G12T substitution under control of prestalk ecmAO promotor in AX2 | axeA2, axeB2, axeC2, rasG(G12T), [pTZ19R], neoR | |
| [ecmA]:GFP | AX4 cells expressing GFP under the control of the prestalk ecmA promoter | axeA1, axeB1, axeC1, [ecmA::GFP], neoR | axe,neoR,wt |
| [ecmA]:lacZ | HL328 (uracil auxotroph) cells expressing lacZ under the control of the prestalk ecmA promoter | axeA1, axeB1, axeC1, pyr5-6-[pdeltaPYR-BglII], ura-, [ecmA::lacZ], neoR | axe, ura-, neoR |
| [ecmA]:lacZ | AX4 expressing lacZ from the prestalk promoter ecmA | axeA1, axeB1, axeC1, [ecmA::lacZ], neoR | axe,neoR |
| [ecmA]:pkaC | pkaC overexpressed under control of the prestalk emcA promotor in AX3 cells | axeA1, axeB1, axeC1, pkaC, neoR | |
| [ecmA]:pkaC(9-648) | PkaC over expressed in AX2 under control of pre-stalk ecmA promotor | axeA2, axeB2, axeC2, pkaC(9-648), [ecmA-pkaC], neoR | |
| [ecmA]:pkaR(G135E/G261A) | constitutively active pkaR (PKA regulatory subunit; point mutant (G135E/G261A) that is unable to bind cAMP under control of ecmA promotor | axeA2, axeB2, axeC2, [ecmA]:pkaR(G135E/G261A), [pEcmA-Rm], neoR | |
| [ecmA]:pkaR(G135E/G261A) | constitutively active pkaR; point mutant (G135E/G261A)
that is unable to bind cAMP under control of ecmA promotor | axeA1, axeB1, axeC1, [ecmA]:pkaR(G135E/G261A), neoR | |
| [ecmA]:pkaR(G135E/G261A)/[ecmB-frag]:lacZ | constitutively active pkaR (PKA regulatory subunit point mutant (G135E/G261A) that is unable to bind cAMP under control of ecmA promotor; coexpressed with lacZ under control of a fragment of the ecmB promotor (-877 to -1) which specifically directs expression in the stalk tube | axeA2, axeB2, axeC2, [ecmA]:pkaR(G135E/G261A), [pEcmA-Rm], lacZ, [pST-gal], neoR | |
| [ecmA]:YFP | DH1 expressing YFP under the control of the prestalk ecmA promoter | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, [pTX-ecmA-YFP], neoR | axe, neoR, ura-, yfp |
| [ecmB]:lacZ | AX2 wild type cells marked with lacZ under the control of the prestalk ecmB promoter | axeA2, axeB2, axeC2, [p56Gal], neoR | axe,neoR |
| [ecmB]:lacZ | AX4 wild type cells marked with lacZ under the control of the prestalk ecmB promoter | axeA1, axeB1, axeC1, [ecmB/lacZ], neoR | |
| [ecmB]:pkaC | pkaC overexpressed under control of the prestalk emcB promotor in AX3 cells | axeA1, axeB1, axeC1, pkaC, neoR | |
| [ecmF]:expl3:myc | myc-tagged expl3 overexpressed in AX2 | axeA2, axeB2, axeC2, expl3:myc, [pLD1ΔBX[ecmF/expl3-myc], neoR | |
| [ecmO]-ile-GFP(S65T) | unstable GFP which does not accumulate with time and thus gives extremely weak fluorescence; directs expression in pstO cells | axeA2, axeB2, axeC2, [ecmO/ile-wt-GFP]. neoR | neoR |
| [expl7]:expl7:GFP | expl7 overexpressor under the control of its own promoter | axeA2, axeB2, axeC2, [expl7/expl7:GFP], neoR | |
| [gpaA]:gpaA | overexpressor of gpaA under the control of its own promoter in a multicopy extrachromosomal vector | axeA1, axeB1, axeC1, [gpaA]:gpaA, neoR | |
| [gpaA]:gpaA(G45V) | overexpressor of gpaA(G45V) (constitutively active mutant) under the control of its own promoter in a multicopy extrachromosomal vector | axeA1, axeB1, axeC1, [gpaA]:gpaA(G45V), neoR | |
| [gpaA]:gpaA(Q206L) | overexpressor of gpaA(Q206L) (constitutively active mutant) under the control of its own promoter in a multicopy extrachromosomal vector | axeA1, axeB1, axeC1, [gpaA]:gpaA(Q206L), neoR | |
| [gpaB]:gpaB(Q208L) | expression of gpaB (G alpha 2) with a point mutation in the GTPbinding/GTPase domain (Q208L) | axeA1, axeB1, axeC1, [gpaB(Q208L)] | |
| [gtaC]:IDQ-gal | gtaC promoter driving the labile IDQ-lacZ | axeA1, axeB1, axeC1, [gtaC]:IDQ-gal, neoR | |
| [hairpin]dct | hairpin construct (dynhp) that silences dynacortin expression | axeA1, axeB1, axeC1, [pREP], [hairpin]dct | |
| [inviable]arcB- | arcB is an essential gene in AX2 as determined by the "stop codon knock-in" method | axeA1, axeB1, axeC1, arcB-, bsR | |
| [inviable]arpB- | haploid blasticidin resistant aprB null mutant cannot be obtained from segregating a diploid arpB-/arpB+ cell line | xeA1, axeB1, axeC1, [PYR5-6-THY1], thy+/-[mycDdarpBpRHI76], bsR | |
| [inviable]cofA- | attempts to produce a cofA null mutant failed; transformants died after 5 days | axeA2, axeB2, axeC2, cofA-, bsR | |
| [inviable]fttB | gene deletion yielded highly enlarged, fragile cells that died after a few days. | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+, fttB-, bsR | |
| [inviable]lkb1- | attempts to produce an lkb1-null mutant failed; RNAi knockdown mutant has severe defects suggesting the gene is essential | axeA1, axeB1, axeC1, lkb1-, bsR | |
| [inviable]phlp2- | attempts to produce a phlp2- null mutant failed; transformants died after 5 days | axeA1, axeB1, axeC1, phlp2-, bsR | |
| [mrrA]:lacZ | lacZ under the control of the mrrA promoter | axeA2, axeB2, axeC2, mrrA:lacZ | |
| [ndm]:ndm:RFP | ndm tagged with C-terminal RFP in AX3 | axeA1, axeB1, axeC1, ndm:RFP, bsr | |
| [OE]:chlim | overexpressor of ChLim | axeA1, axeB1, axeC1, [OE]:chlim, neoR | |
| [OE]:egeA | egeA overexpressed in AX2 | axeA2, axeB2, axeC2, egeA, neoR | |
| [OE]:gbfA | overexpressor of gbfA (G box-binding factor) | axeA1, axeB1, axeC1, unk:gbfA, neoR | |
| [OE]:GFP:gxcA | RacGEF1 overexpressor tagged with GFP at the N terminus | axeA1, axeB1, axeC1, unk:GFP:gxcA | |
| [OE]:gpaD | overexpressor of gpaD (G alpha 4) | axeA1, axeB1, axeC1, [OE]:gpaD | |
| [OE]:gpaD/regA- | regA null mutant in the overexpressor of gpaD (G alpha 4) | axeA1, axeB1, axeC1, [OE]:gpaD, regA-[pregAKO], bsR, neoR | |
| [OE]:gpaE | high copy overexpressor of Gα5 | axeA1, axeB1, axeC1, [pJH206]HC, neoR | axe,neoR,premature tip formation |
| [OE]:HA:rab21 | overexpressor of rab21 with a N-terminal HA tag | axeA1, axeB1, axeC1, [OE]:HA:rab21, neoR | |
| [OE]:HA:rab21(Q66L) | overexpressor of rab21(Q66L) (constitutively active) with a N-terminal HA tag | axeA1, axeB1, axeC1, [OE]:HA:rab21(Q66L), neoR | |
| [OE]:HA:rab21(T21N) | overexpressor of rab21(T21N) (dominant negative) with a N-terminal HA tag | axeA1, axeB1, axeC1, [OE]:HA:rab21(T21N), neoR | |
| [OE]:ipkA1 | ipkA1 overexpression in wild type | axeA2, axeB2, axeC2, ipkA1, bsR | |
| [OE]:itpk1 | itpk1 overexpression in wild type | axeA2, axeB2, axeC2, itpk1, bsR | |
| [OE]:limF | limF overexpressor in AX3 | axeA1, axeB1, axeC1, neoR, [OE]:limF | |
| [OE]:pakC | overexpressor of pakC | axeA1, axeB1, axeC1, [OE]:pakC | |
| [OE]:pdiA | pdi overexpressor controlled by pde late promoter | axeA1, axeB1, axeC1, [pLDI], neoR | axe,neoR,spores at base/thick stalks |
| [OE]:pdiA | pdi overexpressor controlled by pde late promoter | axeA1, axeB1, axeC1, [pLDI], neoR | axe,neoR,spores at base/thick stalks |
| [OE]:pdiA | overexpressor of phosphodiesterase inhibitor from the pde-aggregation promoter | axeA1, axeB1, axeC1, [pADI+pB10TP], neoR | delayed aggregation,neoR,axe |
| [OE]:pdiA | phosphodiesterase-inhibitor overproducer (control plasmid without promoter) | axeA1, axeB1, axeC1, [pCDI+pB10TP], neoR | axe,neoR,wt |
| [OE]:pdiA | phosphodiestrase inhibitor (pdi) overproducer from the pde vegetative promoter | axeA1, axeB1, axeC1, [pVDI+pB10TP], neoR | axe,neoR,wt |
| [OE]:pdsA | Cotransformant of AX3 with pB10T and pGPVC (prV-pde construct containing entire 5prime-untranslated region) | axeA1, axeB1, axeC1, [pB10T+pGPVC], neoR | |
| [OE]:rasB(G12T) | Overexpressor of ras(G12T) (constitutively active) | axeA2, axeB2, axeC2, [OE]:rasB(G12T) | |
| [OE]:sgcA(1-2000)(N1106A):GFP/gca-/sgcA- | overexpressor of sgcA with a mutation replacing the catalytic aspartate at position 1106 with an alanine residue and in which the C terminal 1000 amino acids have been removed, fused to GFP and expressed in sgcA-/gca- cells; the sGC protein remains completely cytosolic | axeA1, axeB1, axeC1, gcA-, sgcA-, unk:sgcA(1-2000)(N1106A):GFP, bsR, hygR | |
| [OE]:sgcA(878-2843)(N1106A):GFP/gca-/sgcA- | overexpressor of sgcA with a mutation replacing the catalytic aspartate at position 1106 with an alanine residue and in which the N terminal 877 amino acids have been removed, fused to GFP and expressed in gca-/sgcA- cells; the sGC protein remains completely cytosolic | axeA1, axeB1, axeC1, gcA-, sgcA-, unk:sgcA(878-2843)(N1106A):GFP, bsR, hygR | |
| [OE]:sgcA(878-2843):GFP/sgcA- | overexpressor of sgcA with a mutation removing the N terminal 877 amino acids, fused to GFP and expressed in gca-/sgcA- cells; the sGC protein remains completely cytosolic | axeA1, axeB1, axeC1, gcA-, sgcA-, unk:sgcA(878-2843):GFP, bsR, hygR | |
| [OE]:sgcA(N1106A):GFP/gca-/sgcA- | overexpressor of sgcA with a mutation replacing the catalytic aspartate at position 1106 with an alanine residue expressed in gca-/sgcA- mutant | axeA1, axeB1, axeC1, gcA-, sgcA-, unk:sgcA(N1106A):GFP, bsR, hygR | |
| [OE]:sgcA:GFP/gca-/sgcA- | sgcA fused to GFP expressed in gca-/sgcA- mutant | axeA1, axeB1, axeC1, gcA-, sgcA-, unk:sgcA:GFP, bsR, hygR | |
| [OE]GFP:gxcA(1-750:764-1217) | RacGEF1-delta IQ overexpressor marked with GFP at the N terminus | axeA1, axeB1, axeC1, unk:GFP:gxcA(1-750:764-1217) | |
| [OE]GFP:gxcA(1-750:764-984) | RacGEF1-deltaIQ/deltaPH overexpressor marked with GFP at the N terminus | axeA1, axeB1, axeC1, unk:GFP:gxcA(1-750:764-984) | |
| [OE]GFP:gxcA(1-984) | RacGEF1-delta PH overexpressor marked with GFP at the N terminus | axeA1, axeB1, axeC1, [OE]GFP:gxcA(1-984) | |
| [OE]GFP:gxcA(568-1217) | RacGEF1-deltaN overexpressor marked with GFP at the N terminus | axeA1, axeB1, axeC1, [OE]GFP:gxcA(568-1217) | |
| [OE]mipp1 | mipp1 overexpression in wild type | axeA2, axeB2, axeC2, mipp1, neoR | |
| [OE]pdiA | pdi overproducer | axeA1, axeB1, axeC1, acrA1, tsgA1, bwn, pdiA | aggregation defective,metR |
| [pdsA-agg]:pdsA | Overexpressor of ePDE (extracellular phosphodiesterase) under the control of its aggregation promoter; pB10T + pGPA co-transformed | axeA1, axeB1, axeC1, [pB10T+pGPA], neoR | axe,neoR |
| [pdsA-ldev]:pdsA | Extracellular phosphodiesterase (ePDE) overexpressor (from its late development promoter). | axeA1, axeB1, axeC1, [pB10T+pGPL], neoR | axe,neoR,no fr. bodies |
| [pirA]:pirA:GFP | insertion of GFP at the C-terminus of the pirA gene | axeA1, axeB1, axeC1, pirA:GFP | |
| [pkaC]:pkaC | pkaC overexpressor, regulated by its endogenous upstream region | axeA2, axeB2, axeC2, [K-Neo], neoR | |
| [pkaC]:pkaC/nsfA(A1854T | temperature sensitive nsfA mutant expressed in pkaC overexpressor cells | axeA2, axeB2, axeC2, [pkaC]:pkaC, [K-Neo], nsfA2(A1854T, T1984A, C2131A, A2398T, T2574A), bsR, neoR | |
| [pkaC]:pkaC/srfA- | srfA null in pkaC overexpressor | axeA2, axeB2, axeC2, pkaC, [pK-Neo], srfA-, [srfA-KO], neoR, bsR | |
| [pkaC]:pkaC/[dscC]:lacZ | lacZ under control of discoidin promotor expressed in pkaC overexpressor | axeA2, axeB2, axeC2, pkaC, [K-Neo], lacZ, neoR, hygR | |
| [psmC1]:psmC1 | psmC1 overexpression under its own promoter in wild type strain | axeA1, axeB1, axeC1, psmC1+ [pEXP4(-)psmC1], neoR | |
| [pspA]-ile-GFP(S65T) | unstable GFP which does not accumulate with time and thus gives relatively weak fluorescence; expressed in prespore cells | axeA2, axeB2, axeC2, [pspA/ile-wt-GFP], neoR | neoR |
