Strain Catalog

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Strain Descriptor Strain Summary Genotype Phenotype
ctxA-/ctxB-/[act15]:acaA:YFPacaA-YFP overexpressed in the ctxA-/ctxB- double null mutantaxeA2, axeB2, axeC2, ctxA-[ctxA-neo], neoR, ctxB-[ctxB-bsr], bsR, acaA-YFP, neoR
ctxA-/ctxB-/[act15]:carA:YFPcarA-YFP overexpressed in the ctxA-/ctxB- double null mutantaxeA2, axeB2, axeC2, ctxA-[ctxA-neo], neoR, ctxB-[ctxB-bsr], bsR, carA-YFP, neoR
ctxA-/ctxB-/[act15]:GFP:arcAGFP-marked ArcA expressed in ctxA-/ctxB- cellsaxeA2, axeB2, axeC2, ctxA-[ctxA-neo], ctxB-[ctxB-bsr], GFP:arcA, [pEXP4(+)], bsR-, neoR
ctxA-/P.pallidumcortexillin I null in P. pallidum with GFPmatI, ctxA-, [pREMI:GFP], neoRneoR
ctxA-/[act15]:enlA(92-497)suppressor strain expressing a truncated enlA (residues 92-497) in ctxA null cells (HS1151)axeA1, axeB1, axeC1, [pREP], ctxA-, enlA(92-497)[pLD1A15SN], neoR
ctxA-/[act15]:GFP-mhcActxA null mutant expressing GFP-mhcA (myosin II heavy chain)axeA2, axeB2, axeC2, ctxA-, neoR, GFP-mhcA, hygR
ctxA-/[RNAi]enlAexpression of a hairpin enlA construct (antisense 1-1483, sense 857-1462) in ctxA null cells (HS1151) to achieve enlA silencing by RNAiaxeA1, axeB1, axeC1, [pREP], ctxA-, enlA(AS1-1483, S857-1462)[pLD1A15SN], neoR
ctxB-ctxB (cortexillin II) null mutantaxeA2, axeB2, axeC2, ctxB-[ctxB-bsr], bsRaxe,bsR
ctxB-ctxB null mutantaxeA2, axeB2, axeC2, ctxB-, bsR
ctxC-ctxC null mutantaxeA1, axeB1, axeC1, ctxC-, [bsRcas], bsR
cudA-cudA-null mutant (MF2)axeA2, axeb2, axeC2, cudA- [cudA-bsr], bsRaxe, bsR
cudA-cudA null mutant; uracil prototrophaxeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, cudA-, ura+
cudA-cudA deletion mutant using pcudAKO(MF2)axeA1, axeB1, axeC1, cudA-, [Mf2], bsR
culA-culA null mutantaxeA1, axeB1, axeC1, culA-, bsR
culA-/[cotC]:culAculA- rescued with culA under the control of the prespore cotC promoter axeA1, axeB1, axeC1, culA-, [cotC/culA], bsR, neoR
culA-/[ecmA]:culAculA- rescued with culA under the control of the prestalk ecmA promoter axeA1, axeB1, axeC1, culA-, [ecmA/culA], bsR, neoR
culA-/[pkaC]pkaCculA- rescued with pkaC under the control of the pkaC promoter axeA1, axeB1, axeC1, culA-, [pkaC/pkaC], bsR, neoR
culB-culB null mutantaxeA1, axeB1, axeC1, culB-, bsR
culB-/pkaR-culB/pkaR double null mutant; deletion of regulatory subunit renders PKA active;axeA1, axeB1, axeC1, culB-, pkaR-, bsR, ?
culB-/[act15]:pkaCculB null mutant overexpressing the catalytic subunit of PKA (pkaC)axeA1, axeB1, axeC1, culB-, pkaC, bsR, neoR?
cybA-cybA null mutant; blasticidin resistantaxeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-, cybA-[pDTa19R], bsR
cynA-cynA null mutant in AX3.1axeA1, axeB1, axeC1, cynA-, [bsRcas], bsR
cyp516A1-cyp516A1 REMI mutant; the insertion site is 277 bp upstream of the stop codon; note, the insertion appears to have deleted a short stretch of genomic DNA;
get raw sequence data		axeA1, axeB1, axeC1, cyp516A1-, bsR
cyp518A1-cyp518A1 REMI mutant; the insertion site is in the second exon, 224 bp upstream of stop codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: attccttGATCaagattctca;
get raw sequence data		axeA1, axeB1, axeC1, cyp518A1-, bsR
cytB-cytB null mutant in AX2axeA2, axeB2, axeC2, cytB-, [pPROF272], neoR
D. versatile MAD 52Natural isolate from Ramonafana National Park, Madagascar; soil sample from humid tropical forestwt
D2B-D2B null mutant in AX2axeA2, axeB2, axeC2, D2B-, bsRcas, bsR
dagA-CRAC null (cytosolic regulator of adenylyl cyclase)axeA1, axeB1, axeC1, dagA-[pBSR1], bsRaxe, agg-, bsR
dagA-dagA null mutantagg-, tsgA1, manA2, axeA1, axeB1, axeC1, acrA+?, bwn+, dagA-agg-,tsg,metR?,axe
dagA-dagA REMI mutantaxeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], ura+, dagA-[DIV2]
dagA-/acaA(L394S)acaA mutant library transformed into dagA (crac) null mutantt resulting in a constitutively active adenylyl cyclase mutantaxeA1, axeB1, axeC1, dagA-[pBSR1], acaA(L394S), bsR, neoRaxe,agg-?,slow growing,bsR?,neoR?
dagA-/[act15]:dagAdagA overexpressor in dagA- backgroundaxeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], ura+, dagA-[DIV2], [act15]:dagA, neoR
dagA-/[act15]:lacZdagA null mutant expressing lacZ under the control of the act15 promoteraxeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], [actin15::lacZ], neoR, dagA-[p120Cla], ura+axe,neoR
dagA-/[cotB]:lacZdagA null mutant expressing lacZ under the control of the cotB promoteraxeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], [cotB::lacZ], neoR, dagA-[p120Cla], ura+
dagA-/[ecmA]:lacZdagA null mutant expressing lacZ under the control of the ecmA promoteraxeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], [ecmA::lacZ], neoR, dagA-[p120Cla], ura+axe, agg-, neoR, lacZ-oxp
darA-darA null mutantaxeA2, axeB2, axeC2, darA-, bsR
DBL 108tester strainaxeA, axeB, nagA108
DBL 78temperature-senstivity of N-acetylglucosaminidase both in vitro and in vivo; haploid tester strainaxeA, axeB, nagA78
DBL17tester strainaxeA, axeB, nagA17
DBL211N-acetylglucosaminidase mutantaxeA1, axeB1, axeC1, nagA211axe,small migrating pseudoplasmodia
DBL230N-acetylglucosaminidase mutantaxeA1, axeB1, axeC1, nagA230axe,small slugs
DBL239-1/HU26heterozygote diploid tester strain; Parents: DBL239-1 and HU26sprA/+, tsgE/+, whiA/+, acrA/+, tsgD12/+, nagA239, cobA/+, tsg
DBL239-1/XM101heterozygote diploid tester strain; Parents: DBL239-1, XM101(DBS0237065)cycA/+, whiA/+, tsgD12/+, acrA1/+, nagA239/+, manA2/+, tsg
DBL251-1/XM101tester heterozygous diploid strain; Parents: DBL251-1,XM101cycA/+, whiA/+, tsgD12/+, acrA1/+, nagA251/+, manA2/+, tsg
DBL251-1/XM101heterozygote diploid tester strain; Parents: DBL251-1 and XM101cycA/+, whiA/+, acrA/+, tsgD12/+, nagA251/+, bwnA1/+, manA2/+, tsg
DBL81-1/XM101heterozygous diploid tester strain; parents: DBL81-1 and XM101cycA/+, whiA/+, tsgD12/+, acrA1/+, nagA81/+, manA2/+, tsg
DC152dd, dc152
dclre1-dclre1 null mutantaxeA2, axeB2, axeC2, dclre1-[[bsR-loxP], bsR
dcsA-dcsA null mutant in HMX44A axe, tsg-901 and/or tsg-903, cyc-900, dif, dcsA-[pdcsA-KO], bsRaxe,agg-,cycR,DIF-,tsg,bsR
dcsA-dcsA REMI mutantaxeA1, axeB1, axeC1, dcsA-[pBSR1], bsRaxe, bsR, gnarled fingers
dcsA-dcsA REMI mutantaxeA1, axeB1, axeC1, dcsA-[pBSR1], bsRaxe, bsR, gnarled fingers
dcsA-dcsA REMI mutantaxeA1, axeB1, axeC1, dcsA-[pBSR1], bsRaxe, bsR, gnarled fingers
dcsA-dcsA null mutantaxeA1, axeB1, axeC1, dcsA-[dcsA-KO], bsRaxe,bsR,blocked at culmination(gnarled fingers)
dcsA-/[act15]:dcsArescue of dcsA null mutantaxeA1, axeB1, axeC1, dcsA-[dcsA-KO], bsR, [act15/dcsA], neoRaxe,bsR,neoR,wt
dct-dct null mutant in AX2axeA2, axeB2, axeC2, dct-, bsRcas, bsR
dcx-/lis1(D327H)dcx-null and lis1-substitution double mutantaxeA2, axeB2, axeC2, dcx-, lis1(D327H), bsR
DD3-3-DD3-3 null mutant; blasticidin resistantaxeA2, axeB2, axeC2, DD3-3-
Dd5P1-Dd5P1 null mutantaxeA1, axeB1, axeC1, pyr5-6-[pRG24], Dd5P1-, ura+
Dd5P1-/Dd5P2-Dd5P1/Dd5P2 double null mutantaxeA1, axeB1, axeC1, pyr5-6-[pRG24], Dd5P1-, Dd5P2-, ura+, bsR
Dd5P1-/Dd5P3-Dd5P1/Dd5P3 double null mutantaxeA1, axeB1, axeC1, pyr5-6-[pRG24], Dd5P1-, Dd5P3-, ura+, bsR
Dd5P2-Dd5P2 null mutantaxeA1, axeB1, axeC1, pyr5-6-[pRG24], Dd5P2-, ura+
Dd5P2-Dd5P2 null mutant; blasticidin resistantaxeA1, axeB1, axeC1, Dd5P2-[Dd5P2-bsr], bsR
Dd5P2-/Dd5P3-Dd5P2/Dd5P3 double null mutantaxeA1, axeB1, axeC1, pyr5-6-[pRG24], Dd5P2-, Dd5P3-, ura+, bsR
Dd5P3-Dd5P3 null mutantaxeA1, axeB1, axeC1, pyr5-6-[pRG24], Dd5P3-, ura+
Dd5P4-Dd5P4 null mutant; blasticidin resistantaxeA1, axeB1, axeC1, Dd5P4-[Dd5P4-bsr], bsR
Dd5P4-/[act15]:cat5P4Dd5P4 null mutant expressing the N-terminal inositol 5-phosphate catalytic domain of Dd5P4 only.axeA1, axeB1, axeC1, Dd5P4-[Dd5P4-bsr], cat5P4, bsR, neoR
Dd5P4-/[act15]:Dd5P4Dd5P4 null mutant expressing wild type Dd5P4axeA1, axeB1, axeC1, Dd5P4-[Dd5P4-bsr], Dd5P4, bsR, neoR
Dd5P4-/[act15]:Dd5P4(D319A):GFPDd5P4 null mutant expressing Dd5P4 with a mutationthat renders the catalytic domaininactive, fused to GFP at the C-terminusaxeA1, axeB1, axeC1, Dd5P4-[Dd5P4-bsr], hsOCRL-D422A, bsR, neoR
Dd5P4-/[act15]:Dd5P4:GFPDd5P4 null mutant expressing wild type Dd5P4 fused to GFP at the C-terminusaxeA1, axeB1, axeC1, Dd5P4-[Dd5P4-bsr], Dd5P4-GFP, bsR, neoR
Dd5P4-/[act15]:GAP5P4Dd5P4 null mutant expressing the C-terminal RhoGAP domain of Dd5P4 only.axeA1, axeB1, axeC1, Dd5P4-[Dd5P4-bsr], GAP5P4, bsR, neoR
Dd5P4-/[act15]:GFP:Dd5P4(D319G)GFP-tagged Dd5P4 with D319G substitution in Dd5P4 nullaxeA1, axeB1, axeC1, Dd5P4-, [Dd5P4-bsr], Dd5P4(D319G), [pDM317], bsR, neoR
Dd5P4-/[act15]:Hs-OCRLDd5P4 null mutant expressing the human ortholog OCRLaxeA1, axeB1, axeC1, Dd5P4-[Dd5P4-bsr], hsOCRL, bsR, neoR
Dd5P4-/[act15]:Hs-OCRL(D422A)Dd5P4 null mutant expressing the human ortholog OCRL with a mutation that renders the catalytic domain inactiveaxeA1, axeB1, axeC1, Dd5P4-[Dd5P4-bsr], hsOCRL-D422A, bsR, neoR
DdBD. discoideum lab strain of M. Sussman, derived from NC4wt
DdBD. discoideum lab strain of Rob Kay (obtained from D. Welker); derived from NC4 and used to create the axenic strain AX1 (since lost), which in turn was used to create strains AX2 (via AX1) and AX3 (and AX4).wt
DDB_G0267692-DDB_G0267692 REMI mutant; the insertion site is 26 bp upstream of the 3 '''' boundary of the 1st exon; insertion at the upper case DpnII restriction site (GATC) in this sequence: ttgattgtGATCcccaaacaggt;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0267692-, bsR
DDB_G0267938-DDB_G0267938 REMI mutant; the insertion site is 1077 bp downstream of the start codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: tcgatgatGATCatgatgatgtt;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0267938-, bsR
DDB_G0267942-DDB_G0267942 null mutant in AX4axeA1, axeB1, axeC1, DDB_G0267942-, bsRcas, bsR
DDB_G0268012-DDB_G0268012 null mutantaxeA2, axeB2, axeC2, DDB_G0268012-
DDB_G0268158-DDB_G0268158 null mutant; PHD zinc finger-containing protein. Total aa/point of insertion: 688/326.axeA1, axeB1, axeC1, bsR, DDB_G0268158-axe, bsR
DDB_G0268222-DDB_G0268222 REMI mutant; the insertion site is 278 bp upstream of the stop codon; insertion at the upper case NlaIII restriction site (CATG) in this sequence: ttcataggCATGagtatggcc;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0268222-, bsR
DDB_G0268328-DDB_G0268328 null mutant (unknown function). Total aa/point of insertion: 3779/918.axeA1, axeB1, axeC1, DDB_G0268328-, bsRaxe, bsR
DDB_G0268678-DDB_G0268678 null mutant; TH1 family protein negative transcription elongation factor NELF subunit. Total aa/point of insertion: 660/249.axeA1, axeB1, axeC1, bsR, DDB_G0268678-axe, bsR
DDB_G0269232-DG1007 REMI mutant; note that the insertion is upstream from the start codonaxeA1, axeB1, DDB_G0269232-, axeC1
DDB_G0269288-DDB_G0269288 REMI mutant; the insertion site is 1159 bp upstream of the stop codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: attggagcaattGATCactaaattcatt
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0269288-, bsR
DDB_G0269308_ps/DDB_G0270470-REMIREMI mutant; note, the insertion site is in an intergenic region; 200 bp upstream of DDB_G0270470, and 697 bp upstream of DDB_G0269308_ps;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0269308_ps-, DDB_G0270470-, bsR
DDB_G0269670-DDB_G0269670 null mutant in AX4axeA1, axeB1, axeC1, DDB_G0269670-, bsRcas, bsR
DDB_G0269760/DDB_G0270940-REMIREMI mutant; note, the insertion site is in an intergenic region; 170 bp upstream from the start codon of gene DDB_G0269760 and 1242 bp upstream of the start codon of DDB_G0270940;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0269760-, DDB_G0270940-, bsR
DDB_G0269834-DDB_G0269834 REMI mutant; the insertion site is 273 bp downstream of the start codon;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0269834-, bsR
DDB_G0270366-DDB_G0270366 null mutant; blasticidin resistantaxeA1, axeB1, axeC1, DDB_G0270366-, bsR
DDB_G0270368-DDB_G0270368 null mutant; blasticidin resistantaxeA1, axeB1, axeC1, DDB_G0270368-, bsR
DDB_G0270586-DDB_G0270586 REMI mutant; the insertion site is 181 bp downstream of the 5'''' boundary of the second exon; insertion at the upper case DpnII restriction site (GATC) in this sequence: gtatatGATCatcatctg;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0270586-, bsR
DDB_G0270636-DDB_G0270636 null mutant; transmembrane protein. Total aa/point of insertion: 1032/426.axeA1, axeB1, axeC1, DDB_G0270636-, bsRaxe, bsR
DDB_G0270652-DDB_G0270652 REMI mutant; the insertion site is 323 bp upstream of the stop codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: tccaatGATCctcttgaaat;
get raw sequence data 		axeA1, axeB1, axeC1, DDB_G0270652-, bsR
DDB_G0270744-DDB_G0270744 null mutant; Rab GTPase domain-containing protein. Total aa/point of insertion: 859/354.axeA1, axeB1, axeC1, bsR, DDB_G0270744-axe, bsR
DDB_G0270786-DDB_G0270786 REMI mutant; the insertion site is 18 bp upstream of the stop codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: atccattGATCatgtaata;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0270786-, bsR
DDB_G0271700-DDB_G0271700 REMI mutant; the insertion site is 263 bp upstream from the stop codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: cttcatcatGATCatgttcaac;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0271700-, bsR
DDB_G0271808-DDB_G0271808 REMI mutant; note, the chromosomal location of the REMI insertion is not available;
get original mutant data		axeA1, axeB1, axeC1, DDB_G0271808-, bsR
DDB_G0272190-DDB_G0272190 null mutantaxeA1, axeB1, axeC1, DDB_G0272190-, bsR
DDB_G0272370-DDB_G0272370 REMI mutant; the insertion site is 138 bp upstream of the stop codon; insertion at the upper case DpnII restriction site (GATC) in this sequence: attagtttaGATCaagattc;
get raw sequence data		axeA1, axeB1, axeC1, DDB_G0272370-, bsR
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