| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| [act15]:dimB:GFP | AX4 cells expressing dimB-GFP under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, dimB:GFPl, neoR | |
| [act15]:dkhC | dhkC overexpressed in KAX3 | axeA1, axeB1, axeC1, dhkC, [pHK12neo], neoR | |
| [act15]:dng1 | dng1 under the control of the act15 promoter | axeA2, axeB2, axeC2, [act15]:dng1, neoR | |
| [act15]:dnmA:myc | C-terminal myc-tagged DNA methyltransferase | axeA2, axeB2, axeC2, [dnmA-C-myc], neoR, bsR | axe,neoR,wt |
| [act15]:drkC(1-497):YFP | C-terminal YFP fusion to drkC where the kinase domain has been deleted expressed in wild type | axeA2, axeB2, axeC2, drkC(1-497)-YFP, [pYU20], neoR | |
| [act15]:dymA:GFP | dymA-GFP expressed in AX2 cells | axeA2, axeB2, axeC2, dymA:GFP, neoR | |
| [act15]:dymB:YFP | dymB tagged with YFP overexpressed in AX2 | axeA2, axeB2, axeC2, dymB:YFP, neoR | |
| [act15]:eif2s1(S53A) | eif2s1 with an serine/alanine substitution at residue 53 expressed in AX4 cells | axeA1, axeB1, axeC1, eif2s1(S53A), [pBsr], bsr | |
| [act15]:eif2s1(S53A)/[bzpR]:lacZ | lacZ under control of the bzpR promotor expressed in eif2s1 cells with an serine/alanine substitution at residue 53 | axeA1, axeB1, axeC1, eif2s1(S53A), [pBsr], lacZ, [pbzpR-9], bsr, neoR | |
| [act15]:eIF6 | eIF6 overexpressed in AX2 | axeA2, axeB2, axeC2, eIF6, [pDXA-3C], neoR | |
| [act15]:empC:GFP | GFP-empC fusion at the c-terminus | axeA2, axeB2, axeC2, [pDEX27]empC:GFP, neoR | |
| [act15]:enlA(92-497) | expression of a truncated enlA (enlazin; residues 92-497) in wild type AX3:REP orf+ cells , acting as a dominant-negative | axeA1, axeB1, axeC1, [pREP], enlA(92-497), [pLD1A15SN], neoR | |
| [act15]:fbxA:FLAG | fbxA FLAG-tagged at the C terminus under the control of the act15 promoter in AX2 | axeA2, axeB2, axeC2, [act15]:fbxA:FLAG, neoR | |
| [act15]:fip(1163-2038) | C-terminal portion of fip expressed in AX4 cells | axeA1, axeB1, axeC1, fip(1163-2038), [pDEX], neoR | |
| [act15]:FLAG:cycC | N-terminal FLAG-tagged CycC overexpressed in AX2 cells under control of the act15 promotor | axeA2, axeB2, axeC2, [act15:FLAG:cycC], neoR | |
| [act15]:FLAG:hbx4 | FLAG-tagged hbx4 overexpressed in KAX3 cells | axeA1, axeB1, axeC1, FLAG:hbx4, [pTX-FLAG], neoR | |
| [act15]:FLAG:hbx4/[act15]:cadA | mutant overexpressing FLAG-tagged hbx4 and rescued by overexpressing cadA | axeA1, axeB1, axeC1, FLAG:hbx4, [pTX-FLAG], cadA, [pEXP4(+)], bsR, neoR | |
| [act15]:FLAG:hbx4/[ecmAO]:lacZ | hxb4 overexpressor expressing lacZ under the control of the prestalk ecmAO promoter | axeA1, axeB1, axeC1, FLAG:hbx4, [pTX-FLAG], [ecmAO]:lacZ, neoR | |
| [act15]:FLAG:mybB | FLAG-tagged mybB under control of act15 promotor expressed in AX2 | axeA2, axeB2, axeC2, FLAG:mybB, [pTX-FLAG], neoR | |
| [act15]:FLAG:ppp4C | FLAG-tagged ppp4C overexpressed in wild type | axeA1, axeB1, axeC1, FLAG: ppp4C, [pEXP4(+)], neoR | |
| [act15]:FLAG:ppp4C(H113Q) | FLAG-tagged ppp4C that contains a H113Q mutation that disrupts phosphatase activity, overexpressed in wild type | axeA1, axeB1, axeC1, FLAG: ppp4C(H113Q), [pEXP4(+)], neoR | |
| [act15]:FLAG:Rab14(Q67L) | constitutively active rab14 in NCA4 cells | axe, FLAG:Rab14(Q67L), neoR | |
| [act15]:FLAG:racE(T25N/E180R/S192D) | constitutively GDP-bound, phospho-mimetic, FLAG-tagged racE(T25N/E180R/S192D) expressed in AX2 | axeA2, axeB2, axeC2, racE(T25N/E180R/S192D), [pTX-GFP], neoR | |
| [act15]:FLAG:rasC(Q62L) | constitutively active rasC(Q62L) in AX2 | axeA2, axeB2, axeC2, rasC(Q62L), pTX-GFP, neoR | |
| [act15]:FLAG:rasC(Q62L/E157R/R164E) | constitutively active rasC(Q62L/E157R/R164E) in AX2 | axeA2, axeB2, axeC2, rasC(Q62L/E157R/R164E), neoR | |
| [act15]:FLAG:sir2A | FLAG-tagged sir2A under control of act15 promotor expressed in AX2 | axeA2, axeB2, axeC2, FLAG:sir2A, [pTX-FLAG], neoR | |
| [act15]:FLAG:sir2C | FLAG-tagged sir2C under control of act15 promotor expressed in AX2 | axeA2, axeB2, axeC2, FLAG:sir2C, [pTX-FLAG], neoR | |
| [act15]:FLAG:sir2D | FLAG-tagged sir2D under control of act15 promotor expressed in AX2 | axeA2, axeB2, axeC2, FLAG:sir2D, [pTX-FLAG], neoR | |
| [act15]:FLAG:wdr45l(A196D) | N-terminal FLAG-tagged wdr45l with A196D substitution in AX4 | axeA1, axeB1, axeC1, wdr45l(A196D), [pDM320], neoR | |
| [act15]:FLAG:wdr45l(G192D) | N-terminal FLAG-tagged wdr45l with G192D substitution in AX4 | axeA1, axeB1, axeC1, wdr45l(G192D), [pDM320], neoR | |
| [act15]:FLAG:wdr45l(T197P) | N-terminal FLAG-tagged wdr45l with T197P substitution in AX4 | axeA1, axeB1, axeC1, wdr45l(T197P), [pDM320], neoR | |
| [act15]:fps | overexpression of fps under the control of the actin 15 promoter; blasticidin resistant | axeA2, axeB2, axeC2, [act15]:fps, bsR | |
| [act15]:fps:GFP | fps marked with C-terminal GFP expressed in AX2 cells | axeA2, axeB2, axeC2, [act15]:fps:GFP, neoR | |
| [act15]:fr1C:GFP | C-terminal GFP-tagged fr1C in AX2 | axeA2, axeB2, axeC2, fr1C, [pDEX-GFPC], neoR | |
| [act15]:fszA:GFP | AX3 cells expressing fszA-GFP under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, Pact15-fszA-GFP, pyr5-6-[pRG24], neoR | axe, neoR |
| [act15]:fszB:GFP | AX3 cells expressing fzsB-GFP under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, Pact15-fszB-GFP, neoR | |
| [act15]:fszB:GFP | GFP-tagged fszB in AX4 | axeA1, axeB1, axeC1, fszB, [pDXA-3C], neoR | |
| [act15]:fttB(K49E):GFP | expression of fttB where the conserved lysine in the ligand-binding cleft has been mutated in wild type background | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], fttB(K49E):GFP, ura+, neoR | |
| [act15]:gbpD | gbpD under the control of the act15 promoter; neomycin resistant | axeA1, axeB1, axeC1, [act15/gbpD], neoR | |
| [act15]:gcsA | gcsA overexpressed in KAX3 | axeA1, axeB1, axeC1, gcsA, [pExp4(+)], neoR | |
| [act15]:GFP | AX2 with GFP expressed from actin15 promoter | axeA2, axeB2, axeC2, [pDdA15gfp], neoR | axe, neoR |
| [act15]:GFP | AX3K with GFP expressed from the actin 15 promoter | axeA1, axeB1, axeC1, [V/A15-GFP], neoR | axe, neoR |
| [act15]:GFP | DH1 expressing GFP under the control of the act15 promoter | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, [pTX-GFP], neoR | axe, neoR, ura-, gfp |
| [act15]:GFP | AX4 cells expressing GFP under the control of the constitutive act15 promoter | axeA1, axeB1, axeC1, GFP, [act15:GFP], neoR | |
| [act15]:GFP(S65T)-racG | racG overexpressor tagged with GFP at the amino terminus under the control of the act15 promoter in AX2 | axeA2, axeB2, axeC2, [act15]:GFP(S65T):racG, neoR | |
| [act15]:GFP(S65T)-racG((G12V)1-122:136-201) | racG-delta-123-135 overexpressor tagged with GFP at the amino terminus under the control of the act15 promoter in AX2 | axeA2, axeB2, axeC2, [act15]:GFP(S65T)-racG((G12V)1-122:136-201), neoR | |
| [act15]:GFP(S65T):dng1 | dng1 wih GFP(S65T) fused at the N terminus nder the control ofthe act15 promoter | axeA2, axeB2, axeC2, [act15]:GFP(S65T):dng1, neoR | |
| [act15]:GFP:abpC(1-250):myc | GFP-tagged, actin-binding region of AbpC expressed in AX4 | axeA1, axeB1, axeC1, GFP:abpC(1-250), [pDXA-HC], neoR | |
| [act15]:GFP:abpC(9-148) | N-terminal fusion of GFP to the actin binding domain of abpC (ABP-120, ddFLN) expressed in wild type | axeA2, axeB2, axeC2, GFP:abpC(9-148), [pDXA-GABD], neoR | |
| [act15]:GFP:abpC(9-148) | N-terminal fusion of GFP to the actin binding domain of abpC (ABP-120) expressed in AX2 | axeA2, axeB2, axeC2, GFP:abpC(9-148), [pDXA-GABD], neoR | |
| [act15]:GFP:abpD(1441-1736) | N-terminal fusion of GFP to a COOH-terminal abpD fragment lacking the final two residues (PT), overexpressed in wild type | axeA2, axeB2, axeC2, GFP-abpD(1441-1736), neoR | |
| [act15]:GFP:abpD(1441-1738) | N-terminal fusion of GFP to a COOH-terminal abpD fragment containing the MAD and a short portion of the rod domain, overexpressed in wild type | axeA2, axeB2, axeC2, GFP-abpD(1441-1738), neoR | |
| [act15]:GFP:abpD(1441-1738 | N-terminal fusion of GFP to a COOH-terminal abpD fragment with a P to A mutation in residue 1737 | axeA2, axeB2, axeC2, GFP-abpD(1441-1738, 1737P/A), neoR | |
| [act15]:GFP:abpE | N-terminal GFP-abpE fusion overexpressed in wild type; extrachromosomal vector | axeA2, axeB2, axeC2, GFP-abpE+[GFP-abpE-neo], neoR | |
| [act15]:GFP:abpE(1-130) | expression of a GFP-ADFH fusion, an N-terminal domain of the Dabp1 (abpE) protein, in wild type | axeA2, axeB2, axeC2, ADFH+[ADFH-neo], neoR | axe, neoR |
| [act15]:GFP:abpE(413-481) | expression of the C-terminal abpE SH3 domain as a GFP fusion protein in wild type | axeA2, axeB2, axeC2, SH3+[SH3-neo], neoR | axe, neoR, increased lateral pseudpodia |
| [act15]:GFP:act15 | AX2 cells expressing GFP-actin fusion construct | axeA2, axeB2, axeC2, [pA15-gfp-actin], neoR | axe, neoR, gfp |
| [act15]:GFP:act15 | AX2 wild type cells marked with GFP under the control of the constitutive act15 promoter | axeA2, axeB2, axeC2, [act15]:GFP:act15, neoR | |
| [act15]:GFP:aqpB(1-208 | GFP-tagged aqpB lacking residues 208-219 expressed in AX2 | axeA2, axeB2, axeC2, aqpB(1-208, 219-294), [pTX-GFP], neoR | |
| [act15]:GFP:arpC | AX2 cells expressing a GFP-Arp3 fusion construct
| axeA2, axeB2, axeC2, [GFP(S65T)-Arp3], neoR | axe, neoR, gfp |
| [act15]:GFP:aurK | Expression of GFP-aurK in wild type | axeA2, axeB2, axeC2, aurK- | |
| [act15]:GFP:cbpG | GFP-tagged cbpG overexpressed in KAX3 | axeA1, axeB1, axeC1, cbpG, [pEXP4(+)], neoR | |
| [act15]:GFP:cepL | cepL over expressed with N-terminal GFPin wild type AX2 | axeA2, axeB2, axeC2, GFP:cepL, [pBsr GFP N2], bsR | |
| [act15]:GFP:cepL(1-1114) | cepL residues 1-1114 overexpressed with N-terminal GFP in wild type AX2 | axeA2, axeB2, axeC2, GFP:cepL(1-1114), [pBsr GFP N2], bsR | |
| [act15]:GFP:cepL(1-763) | cepL residues 1-763 overexpressed with N-terminal GFP in wild type AX2 | axeA2, axeB2, axeC2, GFP:cepL(1-763), [pBsr GFP N2], bsR | |
| [act15]:GFP:cepL(763-1114) | cepL residues 763-1114 overexpressed with N-terminal GFP in wild type AX2 | axeA2, axeB2, axeC2, GFP:cepL(763-1114 ), [pBsr GFP N2], bsR | |
| [act15]:GFP:copB | GFP-copB expressing wild type | axeaxeA2, axeB2, axeC2, GFP:copB [pBsr], bsR | |
| [act15]:GFP:cpnA | wild type cells expressing GFP-cpnA under the control of the act15 promoter | axe, matA1, GFP-cpnA [pTX-GFP], neoR | |
| [act15]:GFP:crtA | GFP-calreticulin in AX2 | axeA2, axeB2, axeC2, [GFP-calreticulin in pDEXRH], neoR | wt,axe,neoR |
| [act15]:GFP:ctnnA | ctnnA (vinA) overexpressed in wild type | axeA2, axeB2, axeC2, GFP-ctnnA, neoR | |
| [act15]:GFP:docD | docD over expressed in AX2 cells | axeA2, axeB2, axeC2, GFP:docD, neoR | |
| [act15]:GFP:forF(1-1087) | wild type overexpressing N-terminal GFP tagged forF, lacking the diaphanous-autoregulatory domain (DAD), under the control of the act15 promoter; this renders the forF constitutively active. | axeA2, axeB2, axeC2, GFP:forF, [pDGFP-MCS-Neo], neoR | |
| [act15]:GFP:forH(1-425) | GFP-tagged and C-terminally truncated drf2 (residues 1-425 expressed; FH1 and FH2 domains deleted) overexpressed in wild type | axeA2, axeB2, axeC2, GFP:forH(1-425), neoR | |
| [act15]:GFP:frmB | GFP-tagged frmB overexpressed in KAX3 | axeA1, axeB1, axeC1, frmB, [pEXP4], neoR | |
| [act15]:GFP:gefQ(172-654) | wild type cells expressing GFP-gefQ lacking the first 172 amino acids and the DEP and RasGEF domains | axeA2, axeB2, axeC2, GFP:gefQ(172-654), bsR | |
| [act15]:GFP:gefQ(173-1298) | wild type cells expressing GFP-gefQ lacking the first 172 amino acids | axeA2, axeB2, axeC2, GFP:gefQ(173-1298), neoR | |
| [act15]:GFP:gefQ(173-651 | wild type cells expressing GFP fused to the gefQ RasGEF domain | axeA2, axeB2, axeC2, GFP:gefQ(173-651, 818-1298), neoR | |
| [act15]:GFP:gefQ(683-833) | wild type cells expressing GFP fused to the gefQ DEP domain | axeA2, axeB2, axeC2, GFP:gefQ(683-833), drugR? | |
| [act15]:GFP:gefQ(910-1298) | wild type cells expressing GFP fused to the gefQ RasGEF domain | axeA2, axeB2, axeC2, GFP:gefQ(910-1298), drugR? | |
| [act15]:GFP:gemA | gemA tagged with GFP overexpressed in AX2 | axeA2, axeB2, axeC2, GFP:gemA, neoR | |
| [act15]:GFP:gflB | GFP-gflB over expressed in AX2 | axeA2, axeB2, axeC2, GFP:gflB, [bsR-cas], bsR | |
| [act15]:GFP:gol | gol (golvesin) fused to GFP at the amino terminus; localizes to the endosome and contractile vacuole as untagged protein | axeA2, axeB2, axeC2, [act15/gfp-golvesin], neoR | axe, neoR |
| [act15]:GFP:gol(1-558) | C-terminally deleted GFP-(N)-Δ(559-579)-golvesin expressing cells | axeA2, axeB2, axeC2GFP:gol(1-558), [pDEX], neoR | axe,neoR |
| [act15]:GFP:gpbB | GFP-marked GpbB over-expressed in AX2-214 cells | axeA2, axeB2, axeC2, GFP:gpbB | |
| [act15]:GFP:gtaC(1-467:497-587) | mutant overexpressing GFP fused to the N-terminus of gtaC missing the putative nuclear localization signal (NLS); gtaC can no longer localize to the nucleus. | axeA2, axeB2, axeC2, [act15:GFP:gtaC(1-467:497-587)], hygR | |
| [act15]:GFP:gtaC(229-587) | mutant overexpressing GFP fused to gtaC with an N-terminal truncation (1-228) under the control of the act15 promoter. | axeA2, axeB2, axeC2, [act15:GFP:gtaC(229-587)], hygR | |
| [act15]:GFP:gtaC(281-467:497-587) | mutant overexpressing GFP fused to the N-terminus of gtaC missing the nuclear localization signal (NLS) and residues 1-280 | axeA2, axeB2, axeC2, [act15:GFP:gtaC(281-467:497-587)]hygR | |
| [act15]:GFP:gtaC(281-587) | mutant overexpressing GFP fused to gtaC with N-terminus trucation (1-280) under the control of the act15 promoter. | axeA2, axeB2, axeC2, [act15:GFP:gtaC(281-587)], hygR | |
| [act15]:GFP:gtaC(346-467:497-587) | mutant overexpressing GFP fused to the N-terminus of gtaC missing the nuclear localization signal (NLS) and residues 1-345 | axeA2, axeB2, axeC2, [act15:GFP:gtaC(346-467:497-587)], bsR, hygR | |
| [act15]:GFP:gtaC(346-587) | mutant overexpressing GFP fused to gtaC with N-terminus trucation (1-345) under the control of the act15 promoter. | axeA2, axeB2, axeC2, [act15:GFP:gtaC(346-587), hygR | |
| [act15]:GFP:gtaC(K469 | mutant overexpressing GFP fused N-terminal to gtaC with Lys and Arg residues in the NLS substituted with Ala under the control of the act15 promoter. | axeA2, axeB2, axeC2, [act15:GFP:gtaC[(K469, 470, 494, 496A/R468, 471A)], hygR | |
| [act15]:GFP:hcpA | overexpressor of N-terminal GFP-hcpA fusion under the control of the act15 promoter | axeA2, axeB2, axeC2, hcpA-[GFP-hcpA], neoR | axe, neoR, slow gowth, aberrant anaphase |
| [act15]:GFP:hcpB | N-terminal GFP-hcpB fusion under the control of the act15 promoter; overexpressor | axeA2, axeB2, axeC2, hcpB-[GFP-hcpB], neoR | slow growth |
| [act15]:GFP:impA1 | impA1 under control of act15 promotor expressed in AX2 | axeA2, axeB2, axeC2, impA1, [pTX-GFP], neoR | |
| [act15]:GFP:krsA | krsA fused to GFP at the N-terminus under the control of the constitutive act15 promoter | axeA2, axeB2, axeC2, [pDGFP-krsA-Neo], neoR | |
| [act15]:GFP:leep1 | N-terminal GFP-marked leep1 overexpressen in AX2 | axeA2, axeB2, axeC2, leep1, [pDM317], neoR | |
| [act15]:GFP:limE | expression of limE fused at the N-terminus to GFP, in wild type cells | axeA2, axeB2, axeC2, limE [pDEXRH-limE], neoR | |
| [act15]:GFP:lis1 | GFP-marked lis1 under control of act5 promotor in AX2 | axeA2, axeB2, axeC2, lis1, [pTX-GFP], neoR | |
| [act15]:GFP:lmnB | lmnB overexpressed and labeled with N-terminal GFP; unstable, disappeared after several passages | axeA2, axeB2, axeC2, GFP:lmnB, [plS191], bsR | |
| [act15]:GFP:mhcA(T1823D/T1833D/T2029D)/AX3 | 3xAsp mutant mhcA, which mimicks the fully phosphorylated state, in AX3 | axeA1, axeB1, axeC1, [pREP], GFP:mhcA(T1823D/T1833D/T2029D), [pDRHyg], hygR | |
