| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| VatMpr | vatM promoter replacement mutant; act6 promoter replaces intrinsic vatM promoter | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [pVATM-act6], ura+ | axe |
| VatMpr/[act15]:vatM | VatMpr cells over-expressing vatM under its own promoter | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [pVATM-act6], ura+, [pVMop], neoR | axe,neoR |
| VatMpr/[act15]:vatM:GFP | The promoter replacement mutant VatMpr expressing VatM-GFP | axeA1, axeB1, axeC1, pyr5-6-[pRG24], vatM-GFP, ura+, neoR | axe, neoR, gfp |
| Vector Control | AX3-ORF+ transformed with pDXA3C. | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6], ura+ | axe, wt |
| VI88 (HBW3) | Mutant that expresses discoidin early during exponential growth and accumulates more discoidin protein than the wild type. | axeA2, axeB2, axeC2 | axe,no preaggregation zone |
| vilA- | vilA knockout mutant | axeA2, axeB2, axeC2, vilA-, [bsRcas], bsR | |
| vmp1- | vmp1 null mutant | axeA1, axeB1, axeC1, vmp1-, bsR | |
| vmp1-/[act15]:vmp1(G268L):GFP | vmp1 with G263L substitution expressed in vmp1 null | axeA1, axeB1, axeC1, vmp1-, vmp1(G268L):GFP, [pDM323], bsR, neoR | |
| vps13F- | vps13F null mutant in DH-10 | axeA1, axeB1, axeC1, pyr5-6-, [pRG24], vps13F-, [bsRcas], bsR, ura- | |
| vps46- | vps46 REMI mutant | axeA1, axeB1, axeC1, vps46-, bsR | |
| vwkA- | null mutant of vwkA | axeA2, axeB2, axeC2, vwkA-[pBsr-Nsi-VwkA-KO], bsR | axe,bsR,delayed development |
| vwkA-/[act15]:GFP:mhcA | GFP-marked MhcA expressed in vwkA null cells | axeA2, axeB2, axeC2, vwkA-[pBsr-Nsi-VwkA-KO], GFP:mhcA, [pTX-GFP], bsR, neoR | |
| wacA- | wacA null mutant | axeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], ura+, wacA-[wacA-ura] | |
| wacA- | wacA null mutant (MIP family gene) | axeA1, axeB1, axeC1, wacA-[wacA-bsr], bsR | |
| wag-4 | Defective phosphodiesterase regulation mutant (no inhibitor) | axeA2, axeB2, axeC2, ? | axe |
| warA- | warA null mutant in kAX3 | axeA1, axeB1, axeC1, bsR, warA- | development arrests at slug stage |
| warA- | warA null mutant in JH10 | axeA1, axeB1, axeC1, warA- | development arrests at slug stage |
| warA-/hbx2- | hbx2/warA- double null mutant in JH10 | axeA1, axeB1, axeC1, bsR, hbx2-, warA- | |
| wasA- | wasA null mutant in AX3 | axeA1, axeB1, axeC1, wasA-, [bsRcas], bsR | |
| wasA-/[tet]:scrA/[unk]:GFP:wasA(I173A/F179A) | GFP-marked wasA(I173A/F179A)?expressed in a wasA- null mutant in which scrA expression is controlled by the Tet-OFF sytem | axeA1, axeB1, axeC1, wasA-, [bsRcas], [tet]:scrA, wasA(I173A/F179A), bsR | |
| wasA-/[unk]:GFP:wasA(I173A/F179A) | wasA(I173A/F179A) expressed in wasA null mutant | axeA1, axeB1, axeC1, wasA-, [bsRcas], wasA(I173A/F179A), bsR | |
| wasA1- | Two copies of the wasA gene had been detected, one copy has been deleted | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, wasA1-, thy+ | |
| wasA1- | hypomorphic strain; two copies of the wasA gene had been detected, one copy has been deleted | axeA2, axeB2, axeC2, wasA1-, wasA2, bsR | delayed development |
| wasA1-/[act15]:GFP:wasA | N-terminal GFP to WASP fusion, separated by the flexible linker GSGSG, under the control of the act15 promoter in the hypomorpic wasA1- strain | axeA2, axeB2, axeC2, [act15]:GFP:wasA, neoR, wasA1-, bsR | |
| wasA1-/[tet]:YFP:wasA2 | very low YFP-WASP expression controlled by a tetracyclin-regulated promoter/transcription activator combination (Tet-OFF), in a background where one copy of the gene has been deleted
| axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, wasA1-, thy+, [tet]:YFP:wasA2, bsR | aggregation defective |
| wasA1-/[tet]:YFP:wasA2/[act15]:wasA | WASP overexpressed in a strain with very low YFP-WASP expression controlled by the Tet-OFF sytem, in a background where one copy of the gene had been deleted | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, wasA1-, thy+, [tet]:YFP:wasA2, bsR, [act15]wasA, neoR | |
| wasA1-/[tet]:YFP:wasA2/[act15]:wasA(Y33A/W39A/F90A/F91A) | WASP aromatic triad mutation in the WH1 domain; an extra F to A mutation at position 91 has ben introduced because of its proximity to F90. Expressed in a strain with very low YFP-WASP expression controlled by the Tet-OFF sytem, in a background where one copy of the gene had been deleted | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, wasA1-, thy+, [tet]:YFP:wasA2, bsR, [act15]wasA(Y33A/W39A/F90A/F91A), neoR | |
| wasA1-/[tet]:YFP:wasA2/[act15]:wipA | overexpression of wipA in the strain where YFP-wasA expression is controlled by the Tet-OFF sytem in a background where one copy of the wasA gene had been deleted | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, wasA1-, thy+, [tet]:YFP:wasA2, bsR, [act15]:wipA, neoR | |
| wasB- | wasB null mutant in AX3 background | axeA1, axeB1, axeC1, wasB-, [bsRcas], bsR | |
| wasB-/[act15]:YFP:wasB | wasB null mutant overexpressing N-terminal YFP tagged wasB under the control of the act15 promoter | axeA1, axeB1, axeC1, wasB-, [bsRcas], YFP:wasB, [pEXP4(+)], bsR, neoR | |
| wasC- | wasC null mutant | axeA1 axeB1, axeC1, wasC-, [bsRcas], bsR | |
| washc5- | washc5 null mutant | axeA2, axeB2, axeC2, washc5- | |
| washc5- | washc5 (strumpellin) null mutant in AX2 background with the BsR cassette floxed | axeA2, axeB2, axeC2, washc5-, [bsR-loxP], bsR | |
| washc5-/[act15]:GFP:washc5 | washc5 null mutant overexpressing N-terminal GFP tagged washc5 under the control of the act15 promoter | axeA2, axeB2, axeC2, washc5-, [pLPBLP], GFP:washc5, [pDM448], bsR, hygR | |
| washc5-/[act15]:washc5(N457D):GFP | washc5 null mutant expressing GFP-tagged washc5 with a N457D subsitution | axeA2, axeB2, axeC2, washc5-, [pLPBLP], washc5(N457D), [pBsr-C1-GFP], bsR | |
| wdr45l-/AX4 | wdr45l null mutant in AX4 | axeA1, axeB1, axeC1, wdr45l-, [bsR-loxP], bsR | |
| wh-1 | original white spores mutant | whi | white spores |
| wimA- | wimA REMI mutant | axeA1, axeB1, axeC1, wimA-, bsR | |
| wimA- | wimA REMI mutant | axeA1, axeB1, axeC1, wimA-, bsR | |
| WS582 | Dictyostelium discoideum natural isolate from Wisconsin (J. Sutherland); mating type | matA2 (mata355) | wt |
| WS629 | Obtained by Gareth Bloomfield from James Cavender. Published as the holotype of Dictyostelium aureum var. luteolum | wt | |
| wshA- | wshA null mutant with a floxed Bsr cassette | axeA2, axeB2, axeC2, wshA-[BsR-cas], bsR | |
| wshA-/atg1- | atg1 null expressed in wshA null cells | axeA2, axeB2, axeC2, wshA-[loxP], atg1-, [bsR-loxP], bsR | |
| wshA-/fam21- | wshA/fam21 double null mutant | axeA2, axeB2, axeC2, wshA-[wshA::bsr], fam21-, bsR | |
| wshA-/fam21- | wash and fam21 multiple null mutant | axeA2, axeB2, axeC2, wshA-, fam21-, bsR | |
| wshA-/fam21-/[act15]:GFP:fam21 | wshA/fam21 double null mutant expressing GFP:fam21 under control of act15 promotor | axeA2, axeB2, axeC2, wshA-, fam21-, GFP:fam21, [pDM448], bsR, hygR | |
| wshA-/fam21-/[act15]:GFP:wshA | wshA/fam21 double null mutant | axeA2, axeB2, axeC2, wshA-[wshA::bsr], fam21-, bsR | |
| wshA-/[act15]:GFP:wshA | wshA null mutant overexpressing N-terminal GFP tagged wshA under the control of the act15 promoter | axeA2, axeB2, axeC2, wshA-, [pLPBLP], GFP:wshA, [pDM448], bsR, hygR | |
| wshA-/[act15]:GFP:wshA/[act15]:vatB:RFP | wshA- cells expressing GFP-WASH and VatB-mRFP | axeA2, axeB2, axeC2, wshA-[BsR-cas], GFP:wshA, vatB:RFP, [pDM351], bsR, neoR | |
| X11 | tester strain | axeA1, axeB1, axeC1, tsgD12?, acrA1, whi, tsgA1, bwn | axe,wt,brown,tsg,metR |
| X2 | tester strain | bwnA, axeA1, axeB1, axeC1, tsgA1 | axe,brown,tsg |
| X22 | tester strain, derived from NC4 | whi, tsgD12, tsgE13, acrA1, sprA1 | tsg,metR,round spores,white spores |
| X36 | | axeA1, axeB1, axeC1, cycA1, tsgA1, bwn, cobA1 | axe,brown,cobR,cycR,tsg |
| xacA- | xacA null mutant in KAX3 | axeA1, axeB1, axeC1, xacA-, [bsRcas], bsR | |
| xacA- | xacA null mutant | axeA2, axeB2, axeC2, xacA-, [bsRcas], bsR | |
| xacA-/[act15]:xacA(1-320) | xacA null mutant expressing the xacA (DRG) GAP and SH3 domains | axeA2, axeB2, axeC2, xacA-, [bsRcas], xacA(1-320), [pDXA], bsR, neoR | |
| xacA-/[act15]:xacA(779-1335) | xacA null mutant expressing the xacA (DRG) DH1 and DH2 domains, flanking one PH domain | axeA2, axeB2, axeC2, xacA-, [bsRcas], xacA(779-1335), [pDXA], bsR, neoR | |
| XGB1 | Meiotic progeny of a cross between HM597 (a clone of A2cycR) and HM598 (a clone of WS205); cycloheximide resistant; mating type II; additional parent DBS ID: HM598(DBS0351241). | cycR, matB, matC, matD | |
| XGB2 | Meiotic progeny of a three-way cross between HM597 (a clone of A2cycR)and HM598 (a clone of WS205) and HM1524 (matAnull). Inherited mtDNA from HM1524.'' cycloheximide sensitive; mating type II; additional parent DBS IDs : HM598(DBS0351241), HM1524(DBS0351242) | matB, matC, matD | |
| XGB4 | Meiotic progeny of a three-way cross between HM597 (a clone of A2cycR), HM598 (a clone of WS205) and HM1524 (matA null); cycloheximide sensitive; mating type II; additional parent DBS IDs : HM598(DBS0351241), HM1524(DBS0351242) | matB, matC, matD | |
| XGB57 | Meiotic progeny of a cross between HM597 (a clone of A2cycR) and WS2162; mating type III, additional parent DBS IDs: HM597(DBS0351239) | cycR, matS, matT | |
| XGB58 | Meiotic progeny of a cross between two parents: HM598 (a clone of WS205) and WS2162; mating type III; cycloheximide sensitive; additional parent DBS ID: HM598(DBS0351241) | matS, matT | |
| XGB7 | Meiotic progeny of a three-way cross between HM597 (a clone of A2cycR), HM598 (a clone of WS205) and HM1524 (matAnull); Inherited mtDNA from HM1524 and HM597; cycloheximide resistant; mating type II; additional parent DBS IDs : HM598(DBS0351241), HM1524(DBS0351242) | cycR, matB, matC, matD | |
| XGB8 | Meiotic progeny of a three-way cross between HM597 (a clone of A2cycR), HM598 (a clone of WS205) and HM1524 (matAnull); Inherited mtDNA from HM1524; mating type II; cycloheximide sensitive; Additional Parents : HM598(DBS0351241), HM1524(DBS0351242) | matB, matC, matD | |
| XM1 | haploid segregant of the diploid strain DMTV1 | cycA1, acrA1, tsgD12, whiA1, axeB1, manA1 | tsg,cycR,metR |
| XM101 | tester strain | cycA1, , tsgD12, whiA1, catA(acrA), bwnA1, manA2 | cycR,tsg,white spores,metR,brown |
| XMC17 | macrocyst-minus mutant (no fusion) | cycA5, bsgA5, fus- | wt,cycR,bsg |
| XMC2 | macrocyst-minus mutant (partial fusion) | cycA5, bsgA5, fus- | wt,cycR,bsg |
| XMC7 | macrocyst-minus mutant (no fusion) | cycA5, bsgA5, fus- | wt,cycR,bsg |
| XP144 | tester strain | cycA1 or 5, whiA1, acrA2, bsgA5, bwnA1, manA2, cobA1 | brown,cycR,cobR,metR,white spores,bsg |
| XP183 | slugger | cycA5, slgE404, tsgA1, axeB1, manA2, bsgA+ | slugger,tsg,cycR |
| XP189 | slugger | tsgE13, sprA1, whiA1, acrA1, tsgD12, slgA400 | slugger, metR,white spores,round spores |
| XP192 | slugger | cycA5, acrA404, slgG407, bsgA5, tsgK21 | metR,cycR,tsg,bsg,slugger |
| XP212 | haploid segregant | whiA1, acrA2, axeA1, axeB1, axeC1, tsgK21, couA351, cobA1 | axe,wt,metR,white spores,cobR,couS,tsg |
| XP228 | slugger | bsgB500, tsgK+, slgF439, cycA5 | slugger,bsg,cycR |
| XP232 | tester strain | whiA1, acrA2, bsgA5, bwnA1, manA2 | white spores,metR,bsg,brown, |
| XP241 | slugger | whiA, acrA, slgI422, bsgA5, tsgK+ | slugger,bsg,metR,white spores |
| XP243 | slugger | cycA, slgF440, bsgB500, tsgK+ | slugger,bsg,cycR |
| XP258 | slugger | cycA5, whiA1, acrA2, bsgA5, tsgK21, slgJ426 | slugger,bsg,tsg,cycR,metR,white spores |
| XP267 | slugger | slgE410, bsgB500, tsgK+ | slugger,bsg |
| XP317 | slugger mutant | bsgC350, ebrB, acrD, slgJ426, tsgK21 | |
| XP55 | tester strain | cycA5, bsgA5 | wt, cycR, bsg |
| XP55 | tester strain | cycA5, bsgA5 | cycR,bsg |
| XP55 | tester strain | cycA5, bsgA5 | wt, cycR, bsg |
| XP95 | tester strain | whiA1, acrA2, axeA1, axeB1, axeC1, tsgA1, bwnA1, manA2, cobA1 | axe,wt,brown,metR,white spores,cobR |
| XP99 | tester strain | bsgA5, bwnA1, cobA1, cycA1or 5, manA2 | cycR,bsg,brown,cobR |
| xpc- | xpc null mutant | axeA2, axeB2, axeC2, xpc-, bsR | |
| xpf- | xpf null mutant | axeA2, axeB2, axeC2, xpf-, bsR | |
| yakA- | yakA REMI mutant | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, yakA-, pyr5-6, ura+ | |
| yakA- | yakA null with insertion in the middle of the kinase domain | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, yakA-, pyr5-6, ura+ | |
| yakA-/AX3 | protein kinase yakA null mutant in AX3 | axeA1, axeB1, axeC1, yakA-[pBSR3-BglII?], bsR | axe,agg-,bsR,small plaque size |
| yakA-/JH8 | protein kinase yakA null mutant in JH8 | axeA1, axeB1, axeC1, py5-6-[PYR5-6-SalI-linker], ura-, yakA-[yakA/bsr], bsR | axe,agg-,ura-,small plaque size,bsR |
| yakA-/keaA- | keaA underexpressor expressed in yakA null cells | axeA1, axeB1, axeC1, yakA-, keaA-, bsR | |
| yakA-/pkaC- | yakA/pkaC double null mutant | axeA1, axeB1, axeC1, pyr5-6- [pRG24], ura-, yakA-, pyr5-6, ura+, pkaC-, bsR | |
| yakA-/pufA- | yakA-/pufA- double null mutant; isolated as a yakA- suppressor | yakA-, pufA-, bsR | yakA suppressor |
| yakA-/yakA-GFP | yakA null cells expressing YakA-GFP | axeA1, axeB1, axeC1, yakA-[pBSR3], bsR, [YakA-GFP], neoR | axe,bsR,neoR,fruiting on SM/5 |
| yakA-/[crlA]:crlA | low-copy expression of crlA from its own promoter in a yakA null mutant | axeA1, axeB1, axeC1, yakA-, [pBR16-crlA], bsR, neoR | |
| yelA-/AX2 | yelA knockout in AX2 | axeA2, axeB2, axeC2, yelA-, [bsRcas], bsR | |
| yelA-/AX4 | yelA REMI mutant | axeA1, axeB1, axeC1, yelA-[pUC118BsrΔBam], bsR | |
| zak2- | zak2 null mutant | axeA1, axeB1, axeC1, zak2-, bsR | |
